PURIFICATION IN AN ACTIVE FORM OF THE PHAGE-PHI-29 PROTEIN-P4 THAT CONTROLS THE VIRAL LATE TRANSCRIPTION
PURIFICATION IN AN ACTIVE FORM OF THE PHAGE-PHI-29 PROTEIN-P4 THAT CONTROLS THE VIRAL LATE TRANSCRIPTION
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DOI：
10.1093/nar/15.19.7781
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发表时间：
1987-10-12
影响因子：
14.9
通讯作者：
SALAS, M
中科院分区：
文献类型：
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作者：
BARTHELEMY, I;LAZARO, JM;SALAS, M
控制病毒晚期转录的噬菌体ø 29蛋白p4是从携带含基因4质粒的大肠杆菌细胞中高度纯化的。该蛋白质以高度纯化的形式获得，占总细胞蛋白质的约6%。通过氨基酸分析和NH 2-末端序列测定，该蛋白被表征为p4。纯化的蛋白质在体外转录试验中具有活性，在枯草芽孢杆菌α43-RNA聚合酶全酶存在下，允许特异性地起始于p29 A3晚期启动子的转录。
The phage ø29 protein p4, that controls viral late transcription, was highly purified fromEscherichia colicells harbouring a gene 4-containing plasmid. This protein, representing about 6% of the total cellular protein, was obtained in a highly purified form. The protein was characterized as p4 by amino acid analysis and NH2—terminal sequence determination. The purified protein was active in anin vitrotranscription assay, allowing specific initiation of transcription at the ø 29 A3 late promoter in the presence ofBacillus subtilisα43-RNA polymerase holoenzyme.