Prenatal exposure to nicotine disrupts synaptic network formation by inhibiting spontaneous correlated wave activity.
Prenatal exposure to nicotine disrupts synaptic network formation by inhibiting spontaneous correlated wave activity.
复制标题
产前接触尼古丁会通过抑制自发相关波活动来破坏突触网络的形成。
DOI：
10.1016/j.ibror.2020.06.003
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发表时间：
2020
期刊：
影响因子：
2.6
通讯作者：
K.
中科院分区：
文献类型：
--
作者：
Momose-Sato;Y. and Sato;K.
相关的自发活动在中枢神经系统的大范围内传播，在胚胎发育的特定时期表现出来。我们之前使用一种电压敏感染料的光学成像技术证明了这种波状活动，我们称之为去极化波，基本上参与了突触网络形成的早期过程。我们发现，在卵巢中应用荷包牡丹碱/士的宁，阻断了介导该波的神经递质，显著减少了脑干感觉核中功能性突触的表达。这一结果，特别是烟碱型乙酰胆碱受体的拮抗剂Ford-Tubocurarine，表明与母亲吸烟相关的产前尼古丁暴露通过干扰相关波而影响神经回路的形成。在本研究中，我们通过检测尼古丁对相关活动的影响和评估尼古丁对迷走神经感觉通路上功能性突触表达的慢性作用来检验这一假说。在对鸡胚胎行为的体外观察和体外准备中使用的电记录显示，尼古丁的应用瞬时增加了与波相关的胚胎运动和电爆发，但随后抑制了这些活动，表明该药物的主要作用是抑制波。这种电压敏感染料的光学成像显示，长期接触尼古丁显著减少了迷走神经高阶感觉核--臂旁核--功能性突触的表达。结果表明，产前尼古丁暴露通过抑制相关的自发波活动，扰乱了神经回路的初始形成。
Correlated spontaneous activity propagating over a wide region of the central nervous system is expressed during a specific period of embryonic development. We previously demonstrated using an optical imaging technique with a voltage-sensitive dye that this wave-like activity, which we referred to as the depolarization wave, is fundamentally involved in the early process of synaptic network formation. We found that thein ovoapplication of bicuculline/strychnine ord-tubocurarine, which blocked the neurotransmitters mediating the wave, significantly reduced functional synaptic expression in the brainstem sensory nucleus. This result, particularly ford-tubocurarine, an antagonist of nicotinic acetylcholine receptors, suggested that prenatal nicotine exposure associated with maternal smoking affects the development of neural circuit formation by interfering with the correlated wave. In the present study, we tested this hypothesis by examining the effects of nicotine on the correlated activity and assessing the chronic action of nicotinein ovoon functional synaptic expression along the vagal sensory pathway.In ovoobservations of chick embryo behavior and electrical recording usingin vitropreparations showed that the application of nicotine transiently increased embryonic movements and electrical bursts associated with the wave, but subsequently inhibited these activities, suggesting that the dominant action of the drug was to inhibit the wave. Optical imaging with the voltage-sensitive dye showed that the chronic exposure to nicotinein ovomarkedly reduced functional synaptic expression in the higher-order sensory nucleus of the vagus nerve, the parabrachial nucleus. The results suggest that prenatal nicotine exposure disrupts the initial formation of the neural circuitry by inhibiting correlated spontaneous wave activity.