MEASUREMENT OF THE SCOTOPIC PIGMENT IN THE LIVING HUMAN EYE
MEASUREMENT OF THE SCOTOPIC PIGMENT IN THE LIVING HUMAN EYE
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DOI：
10.1113/jphysiol.1955.sp005399
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发表时间：
1955-01-01
影响因子：
5.5
通讯作者：
RUSHTON, WAH
中科院分区：
文献类型：
--
作者：
CAMPBELL, FW;RUSHTON, WAH
设计了一种测量视紫红质密度的技术。它使用的原理是，当眼底是看到在检眼镜，它是看到光已通过两次通过视网膜。如果观察光是绿色，它将被视网膜视紫红质吸收而衰减，因此通过测量它可以估计视紫红质的量。测量需要7秒或更短的时间;视紫红质密度的变化可以在整个适应过程中进行。在光中密度福尔斯下降，光越强下降越快。它在黑暗中又渐渐升起。Bunsen-Roscoe定律适用于长达48秒的曝光漂白。如果使用单色漂白灯，如果这些灯先前已被调整为具有相同的暗视亮度，则每种颜色的效果都是相同的。对视网膜的测量表明，在中央凹或视盘处没有漂白效应;任何区域的效应大小都以一种特有的方式取决于与中央凹的距离。以前已经发现了类似的依赖性的分布杆密度和绝对阈值的视觉。
A technique is devised to measure the density of rhodopsin. It uses the principle that when the fundus oculi is seen in an ophthalmoscope, it is seen by light which has passed twice through the retina. If the viewing light is green it will be attenuated from absorption by retinal rhodopsin, so by measuringing it the amount of rhodopsin may be estimated. Measurement takes 7 seconds or less; the change in rhodopsin density may be followed throughout the course of adaptation. In the light the density falls, the stronger the light the more rapid the fall. It rises again gradually in the dark. The Bunsen-Roscoe law holds for bleaching for exposures up to 48 seconds. If monochromatic bleaching lights are used, the effect of every color is the same if the lights have previously been adjusted to have the same scotopic brightness. Measurements of the retina show that there is no effect of bleaching at the fovea or optic disk; that the size of the effect in any region depends in a characteristic way upon the distance from the fovea. Similar dependence has previously been found for the distribution of rod density and the absolute threshold of vision.