The Protective Effect of Rutin against Ischemia/reperfusion-associated Hemodynamic Alteration through Antioxidant Activity
The Protective Effect of Rutin against Ischemia/reperfusion-associated Hemodynamic Alteration through Antioxidant Activity
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DOI：
10.1007/s12272-012-0617-6
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发表时间：
2012-06-01
影响因子：
6.7
通讯作者：
Chae, Han-Jung
中科院分区：
文献类型：
--
作者：
Bhandary, Bidur;Piao, Cheng Shi;Chae, Han-Jung
活性氧在多种器官的缺血再灌注（I/R）损伤中发挥毒性作用。本研究旨在评价鹰嘴豆芽素A、大豆苷元、染料木黄酮、芦丁和槲皮素等对大豆枯萎病的预防作用。这些化合物是众所周知的天然存在的化合物，具有有益的健康效果和抗氧化活性。采用1，1-二苯基-2-苦基肼（DPPH）法和超氧化物歧化酶（SOD）法测定其清除自由基的活性。结果表明，鹰嘴豆芽素A、槲皮素和芦丁具有明显的清除DPPH自由基的活性。类似地，鹰嘴豆芽素A，染料木素和芦丁的治疗显着增加SOD活性，在体外培养的大鼠心肌细胞原代细胞，以及在H9 C2细胞。对于离体研究，来自Sprague-Dawley大鼠的心脏在Langendorff装置中用具有95%O-2和5%CO2的气体混合物的Krebs-Henseleit溶液灌注。使心脏经受20分钟的预缺血，随后是20分钟的全缺血，然后在37 ℃下再灌注50分钟。在缺血前10分钟和整个再灌注期间灌注测试化合物。在所有受试药物中，只有芦丁显著增加左心室发展压（LVDP）和增加最大正和负dP/dt（+/- dP/dtmax）。在左室舒张末压（LVEDP）分析中，芦丁、大豆苷元和鹰嘴豆芽素A有效。芦丁通过影响心肌动力学因素及提高SOD、DPPH活性对缺血再灌注损伤具有保护作用。
Reactive oxygen species exert toxic effects during ischemia-reperfusion (I/R) injury of various organs. This study was designed to evaluate the preventive effects of various isoflavonoids such as biochanin A, daidzein, genistein, rutin and quercetin. These compounds are well-known naturally occurring compounds with beneficial health effects and antioxidant activity. Free radical scavenging activity was measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide dismutase (SOD) assay. Among the isoflavonoids tested, biochanine A, quercetin and rutin showed significant DPPH free radical scavenging activity. Similarly, treatment of biochanine A, genistein and rutin significantly increased SOD activity in neonant rat heart myocyte primary cells as well as in H9C2 cells. For ex vivo study, hearts from Sprague-Dawley rats were perfused in Langendorff apparatus with Krebs-Henseleit solution with a gas mixture of 95% O-2 and 5% CO2. Hearts were subjected to 20 min of pre-ischemia followed by 20 min of global ischemia, and then 50 min of reperfusion at 37 degrees C. The test compounds were perfused 10 min before ischemia and during the entire reperfusion period. Among the isoflavonoids tested, only rutin significantly increased left ventricular developed pressure (LVDP) and increased maximum positive and negative dP/dt (+/- dP/dtmax). In left ventricular end diastolic pressure (LVEDP) analysis, rutin, daidzein and biochanin A were effective. Among the isoflavonoids, rutin had consistent protective effects in I/R injury by affecting cardiac dynamic factors as well as by enhancing SOD and DPPH activity.