Screening of Mothers in a COVID-19 Low-Prevalence Region: Determination of SARS-CoV-2 Antibodies in 401 Mothers from Rostock by ELISA and Confirmation by Immunofluorescence
Screening of Mothers in a COVID-19 Low-Prevalence Region: Determination of SARS-CoV-2 Antibodies in 401 Mothers from Rostock by ELISA and Confirmation by Immunofluorescence
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DOI：
10.1055/a-1197-4293
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发表时间：
2020-08-01
影响因子：
0.6
通讯作者：
Emmerich, Petra
中科院分区：
文献类型：
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作者：
Reisinger, Emil C.;von Possel, Ronald;Emmerich, Petra
背景 与成人相比，儿童感染 SARS-CoV-2（COVID-19 的病因）往往临床上不明显，严重程度也较低。这种感染在儿童中的传播对脆弱的成年人构成了危险。因此，托儿所和学校目前普遍关闭。 方法 由于母亲和孩子的感染状况往往是一致的，我们对罗斯托克市（德国东北部梅克伦堡-威斯特波美拉尼亚州）的 401 名 1 至 10 岁儿童的母亲进行了咽拭子中 SARS-CoV-2 RNA 的检测，以及血清中 SARS-CoV-2 抗体的检测。在咽拭子中检测到该病毒的 RNA。 ELISA检测中，1份血清样本中IgG抗体呈阳性，11份血清样本中IgA抗体呈阳性，3份血清样本呈临界值。所有 401 份血清在使用 FITC 标记的 IgG、IgA 和 IgM 抗体进行的间接免疫荧光测试 (IIFT) 中均呈阴性。结论 在本研究进行时，在罗斯托克接受测试的母亲中，既未检测到 SARS-CoV-2 RNA，也未检测到针对 SARS-CoV-2 的特异性抗体。
Background In children, the infection with SARS-CoV-2, the cause of COVID-19, tends to be clinically inapparent more often or less severe than in adults. The spread of this infection from children poses a danger to vulnerable adults. Therefore, child care institutions and schools currently are widely closed.Methods Since the status of infection tends to be congruent in mothers and their children, we tested 401 mothers of children between 1 and 10 years in the city of Rostock (State of Mecklenburg-Westpomerania, northeast of Germany), for the presence of RNA of SARS-CoV-2 in throat swabs, and of antibodies against SARS-CoV-2 in serum.Results In none of the mothers tested, RNA of this virus was detected in the throat swab. In the ELISA test, IgG antibodies were positive in one serum sample, IgA antibodies were positive in 11, and borderline in 3 serum samples. All 401 sera were negative in the indirect immunofluorescence test (IIFT) with FITC-labeled IgG, IgA, und IgM antibodies.Conclusion At the time of this study, neither SARS-CoV-2 RNA, nor specific antibodies against SARS-CoV-2 were detectable in the mothers tested in Rostock.