REGULATION OF EQUINE HERPESVIRUS TYPE-1 GENE-EXPRESSION - CHARACTERIZATION OF IMMEDIATE EARLY, EARLY, AND LATE TRANSCRIPTION
REGULATION OF EQUINE HERPESVIRUS TYPE-1 GENE-EXPRESSION - CHARACTERIZATION OF IMMEDIATE EARLY, EARLY, AND LATE TRANSCRIPTION
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DOI：
10.1016/0042-6822(87)90240-6
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发表时间：
1987-05-01
期刊：
影响因子：
3.7
通讯作者：
STACZEK, J
中科院分区：
文献类型：
--
作者：
GRAY, WL;BAUMANN, RP;STACZEK, J
用代谢抑制剂在感染的兔肾细胞中检测了马疱疹病毒1型（EHV-1）转录的调节。为了绘制EHV-1即刻早期、早期和晚期转录物的图谱，将病毒RNA在体内进行32 P标记，并与固定在硝酸纤维素滤膜上的EHV-1 DNA限制性片段杂交。立即早期病毒RNA被映射到反向重复DNA序列内的病毒基因组的一个区域（映射单位0.78-0.83和0.95-1.01）。使用来自该区域的32 P标记的克隆DNA探针的北方印迹杂交分析鉴定了单个立即早期病毒转录物（约6 kb）。早期和晚期基因的转录不限于病毒基因组上的任何特定区域，如32 P标记的早期和晚期RNA与来自EHV-1 DNA的长组分和短组分的EHV-1限制性内切酶片段杂交的能力所示。在不使用代谢抑制剂的情况下进行的其他实验证实，EHV-1转录受到时间调节。EHV-1在生产性感染过程中转录的特征将作为分析致癌转化和持续感染细胞中病毒1转录物的参考。
The regulation of equine herpesvirus type 1 (EHV-1) transcription was examined in infected rabbit kidney cells using metabolic inhibitors. In order to map EHV-1 immediate early, early, and late transcripts, viral RNA was 32P-labeled in vivo and hybridized to EHV-1 DNA restriction fragments immobilized on nitrocellulose filters. Immediate early viral RNA was mapped to one region of the viral genome within the inverted repeat DNA sequences (map units 0.78-0.83 and 0.95-1.01). Northern blot hybridization analysis using a 32P-labeled cloned DNA probe from this region identified a single immediate early viral transcript (approximately 6 kb). Transcription of early and late genes was not restricted to any specific region on the viral genome as indicated by the ability of 32P-labeled early and late RNA to hybridize to EHV-1 restriction endonuclease fragments from both the long and short components of EHV-1 DNA. Additional experiments performed without the use of metabolic inhibitors confirmed that EHV-1 transcription is temporally regulated. The characterization of EHV-1 transcription during productive infection will serve as a reference for the analysis of virl transcripts in oncogenically transformed and persistently infected cells.