Cyclooxygenase 2 is induced in colonic epithelial cells in inflammatory bowel disease
Cyclooxygenase 2 is induced in colonic epithelial cells in inflammatory bowel disease
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DOI：
10.1016/s0016-5085(98)70196-9
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发表时间：
1998-08-01
期刊：
影响因子：
29.4
通讯作者：
Stenson, WF
中科院分区：
文献类型：
--
作者：
Singer, II;Kawka, DW;Stenson, WF
背景与目的：前列腺素是由环氧合酶-1和环氧合酶-2合成的。研究环氧合酶-1和环氧合酶-2在正常人结肠和炎症性肠病(IBD)手术切除组织中的表达及细胞定位。方法：用Western blotting和免疫组织化学方法检测COX-1和COX-2蛋白的表达和细胞定位。结果：COX-1蛋白在正常、克罗恩病和溃疡性结肠炎结肠上皮细胞中的表达水平相同。COX-2蛋白在正常肠上皮细胞中未见表达，而在克罗恩病和溃疡性结肠炎上皮细胞中均有表达。正常结肠炎、克罗恩结肠炎和溃疡性结肠炎组织的免疫组织化学显示，COX-1在结肠隐窝下半部的上皮细胞中表达相同。COX-2在正常结肠组织中不表达，而在克罗恩结肠炎和溃疡性结肠炎组织中，COX-2在结肠根尖上皮细胞和固有层单核细胞中表达。在克罗恩回肠炎中，COX-2表达于绒毛上皮细胞。在溃疡性结肠炎中，表达COX-2的结肠上皮细胞也表达诱导型一氧化氮合酶。结论：COX-1在正常回肠和结肠隐窝上皮细胞中均有表达，在IBD中表达无明显变化。COX-2在正常回肠和结肠中未见表达，但在IBD炎症灶的顶端上皮细胞中有表达。
Background & Aims: Prostaglandins are synthesized by cyclooxygenases (COX)-1 and -2. The expression and cellular localization of COX-1 and COX-2 in normal human colon and inflammatory bowel disease (IBD) surgical resections were studied. Methods: COX-1 and COX-2 protein expression and cellular localization were assessed by Western blotting and immunohistochemistry. Results: COX-1 protein was expressed at equal levels in normal, Crohn's disease, and ulcerative colitis colonic epithelial cells. COX-2 protein was not detected in normal epithelial cells but was detected in Crohn's disease and ulcerative colitis epithelial cells. Immunohistochemistry of normal, Crohn's colitis, and ulcerative colitis tissue showed equivalent COX-1 expression in epithelial cells in the lower half of the colonic crypts. COX-2 expression was absent from normal colon, whereas in Crohn's colitis and ulcerative colitis, COX-2 was observed in apical epithelial cells and in lamina propria mononuclear cells. In Crohn's ileitis, COX-2 was present in the villus epithelial cells. In ulcerative colitis, colonic epithelial cells expressing COX-2 also expressed inducible nitric oxide synthase. Conclusions: COX-1 was localized in the crypt epithelium of the normal ileum and colon, and its expression was unchanged in IBD. COX-2 was undetectable in normal ileum or colon, but it was induced in apical epithelial cells of inflamed foci in IBD.