Expression of cartilage oligomeric matrix protein by human synovium
Expression of cartilage oligomeric matrix protein by human synovium
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DOI：
10.1016/s0014-5793(97)00789-8
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发表时间：
1997-07-21
期刊：
影响因子：
3.5
通讯作者：
Perris, R
中科院分区：
文献类型：
--
作者：
DiCesare, PE;Carlson, CS;Perris, R
对人滑膜进行了软骨低聚基质蛋白(COMP)的可能表达分析，COMP多克隆抗血清免疫染色在滑膜细胞和邻近结缔组织中呈阳性染色，在深层结缔组织中染色较弱，用抗COMP的多克隆或单抗进行免疫印迹分析，证实了COMP的存在，免疫反应条带与纯化的关节软骨COMP的分子质量相同(约110 kDa)。用跨越人类COMP外显子7-13的寡核苷酸进行聚合酶链式反应，发现从人软骨细胞或滑膜制备的cDNA扩增产物相同。用生物素标记的探针对人COMP进行Northern印迹分析，也发现了与人软骨细胞或滑膜总RNA相同的杂交产物，在任何软骨代谢生物标志物的研究中，人类滑膜都应该被认为是COMP的潜在组织来源。(C)1997年欧洲生化学会联合会。
Human synovium was analyzed for the possible expression of cartilage oligomeric matrix protein (COMP), Immunostaining with polyclonal antiserum to COMP demonstrated positive staining within the synovial cells and immediately subjacent connective tissue, with less intense staining in the deeper connective tissue, Western blot analysis using either polyclonal or monoclonal antibodies to human COMP confirmed the presence of COMP by immunoreactive bands with the same molecular mass (approximately 110 kDa) as purified articular cartilage COMP. PCR using oligonucleotides that span human COMP exons 7-13 revealed identical amplification products from cDNA prepared from either human chondrocytes or synovium. Northern blot analysis using a biotinylated-probe to human COMP, spanning exons 12-13, also reveal an identical hybridization product to either human chondrocyte or synovium total RNA, Human synovium should be considered as a potential tissue source of COMP in any investigation of biological markers of cartilage metabolism. (C) 1997 Federation of European Biochemical Societies.