Molecular and functional analysis of Caenorhabditis elegans CHIP, a homologue of Mammalian CHIP
Molecular and functional analysis of Caenorhabditis elegans CHIP, a homologue of Mammalian CHIP
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DOI：
10.1016/j.febslet.2004.03.084
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发表时间：
2004-05-01
期刊：
影响因子：
3.5
通讯作者：
Nukina, N
中科院分区：
文献类型：
--
作者：
Khan, LA;Nukina, N
最近发现的Hsc 70相互作用蛋白（CHIP）的C-末端分子是一种E3泛素连接酶，它与分子伴侣合作降解错误折叠或未折叠的蛋白质。CHIP在动物和植物发育中的生理作用在很大程度上仍然未知。在这里，我们表明，敲低CeCHIP的RNAi和敲除的缺失突变逮捕了发展的动物在幼虫阶段。CeCHIP在所有组织中普遍表达，但存在表达水平的组织特异性变化。CeCHIP在体内产生剂量依赖型。CHIP的过表达导致胚胎死亡，而相对较低水平的过表达导致运动和产卵缺陷，并且CHIP过表达的动物在较高温度下形成dauers。(C)2004年由Elsevier B. V.代表欧洲生物化学学会联合会出版。
A recently identified molecule C-terminus of Hsc70 interacting protein (CHIP) has been reported to be an E3 ubiquitin ligase collaborating with molecular chaperones for the degradation of misfolded or unfolded proteins. The physiological roles of CHIP in animal and plant development remain largely unknown. Here, we show that the knockdown of CeCHIP by RNAi and knockout by a deletion mutation arrests the development of the animal at the larval stage. CeCHIP expresses ubiquitously in all tissues but there are tissue specific variations of expression level. CeCHIP produces dose dependent phenotypes in vivo. Over expression of CHIP causes embryonic lethality, while a comparatively lower level of over expression causes locomotion and egg laying defects, and the CHIP over expressed animals form dauers at a higher temperature. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.