Utilization of a Wheat55K SNP array-derived high-density genetic map for high-resolution mapping of quantitative trait loci for important kernel-related traits in common wheat
Utilization of a Wheat55K SNP array-derived high-density genetic map for high-resolution mapping of quantitative trait loci for important kernel-related traits in common wheat
复制标题
利用 Wheat55K SNP 阵列衍生的高密度遗传图谱对普通小麦重要的籽粒相关性状的数量性状位点进行高分辨率作图
DOI：
10.1007/s00122-020-03732-8
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发表时间：
2021-01-03
影响因子：
5.4
通讯作者：
Li, Zhi
中科院分区：
文献类型：
--
作者：
Ren, Tianheng;Fan, Tao;Li, Zhi
本研究利用高密度遗传图谱定位了与籽粒相关性状相关的QTL。利用小麦55 K单核苷酸多态性基因芯片对川农18与T1208杂交构建的重组自交系群体中的371个株系进行了基因型分析。构建了一张由11,583个标记组成的小麦高密度遗传图谱，分子量为4192.62cM，分布于21条染色体上。在多个环境中定位了重要籽粒相关性状的QTL。利用ICIM法和MET法分别定位了96个和151个QTL。在21条染色体上共检测到114个双基因上位性QTL，并分析了各性状的上位性效应。BLAST分析表明，首次定位了23个与籽粒相关性状相关的QTL，其中5个为与籽粒直径比相关的主效稳定QTL（4 BS上为121.34-126.83 cM），每平方米的穗数穗粒重（2DS）为71.32 -75.26 cM，粒长（6A为16.78-31.64 cM，3D为51.63-58.40 cM）。共检测到15个QTL簇，包含58个QTL，所有最稳定的QTL都包含在这些QTL簇中。不同性状间存在显著相关，并进行了讨论。这些结果为进一步定位和克隆稳定QTL的相关基因奠定了基础。
Key messageThis study mapped QTLs associated with kernel-related traits by high-density genetic map. Five new major and stable QTLs for KL, KDR, SN, and KWPS were mapped in multiple environments.AbstractIn the present study, a recombinant inbred line population including 371 lines derived from the cross of Chuannong18 and T1208 was genotyped using the Wheat55K single nucleotide polymorphism array. A novel high-density genetic map consisting of 11,583 markers spanning 4192.62 cM and distributed across 21 wheat chromosomes was constructed. QTLs for important kernel-related traits were mapped in multiple environments. A total of 96 and 151 QTLs were mapped by using the ICIM method and the MET method, respectively. And a total of 114 digenic epistatic QTLs were also detected across 21 chromosomes, and the epistatic effects of each trait were analyzed. BLAST analysis showed that 23 QTLs for different kernel-related traits were first time mapped and five of them were major and stable QTLs for kernel diameter ratio (121.34–126.83 cM on 4BS), spike number per square meter (71.32–73.84 cM on 2DS), kernel weight per spike (71.32–75.26 cM on 2DS), and kernel length (16.78–31.64 cM on 6A and 51.63–58.40 cM on 3D), respectively. Fifteen QTL clusters that contained 58 QTLs were also detected, and all most stable QTLs were contained in these QTL clusters. Significant correlations between different traits were detected and discussed. These results lay the foundation for fine mapping and cloning of the gene(s) underlying the stable QTLs detected in this study.