Bone marrow-derived lymphoid cell lines from patients with agammaglobulinemia.
Bone marrow-derived lymphoid cell lines from patients with agammaglobulinemia.
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来自无丙种球蛋白血症患者的骨髓来源的淋巴细胞系。
DOI：
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发表时间：
1978
影响因子：
15.9
通讯作者：
F. Rosen
中科院分区：
文献类型：
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作者：
J. Schwaber;H. Lazarus;F. Rosen
用EB病毒体外感染X连锁或常见变异无丙种球蛋白血症患者的骨髓来源（B）淋巴细胞，建立了长期B淋巴细胞系（LCL）。仅用来自B细胞对体外促有丝分裂刺激无反应的患者的B淋巴细胞建立LCL。相反，来自没有小B淋巴细胞或具有合成但不能分泌IG的B细胞的患者的淋巴细胞均不能建立LCL。对“非分泌性”B淋巴细胞的分析表明存在C3 B和C3 d受体以及表面IG，但不存在可检测的EB病毒受体。建立的所有六种细胞系与EAC 3形成了红细胞，但与绵羊红细胞不形成红细胞。6个LCL中的4个主要是表面IgD承载的，并且不合成可检测的IG用于分泌。其中两个细胞系与正常个体的细胞系无法区分：它们具有表面IgG、A、M和D，并合成和分泌IG。
In vitro infection with Epstein-Barr virus of bone marrow-derived (B) lymphocytes from blood of patients with X-linked or common varied agammaglobulinemia resulted in the establishment of long-term B-lymphoid cell lines (LCL). LCL were established only with B lymphocytes from patients whose B cells failed to respond to in vitro mitogenic stimulation. In contrast, lymphocytes from patients without small B lymphocytes or with B cells that synthesized but failed to secrete Ig failed uniformly to establish LCL. Analysis of the "nonsecretory" B lymphocytes demonstrated the presence of receptors for C3b and C3d as well as surface Ig, but absence of detectable receptors for Epstein-Barr virus. All of the six cell lines that were established formed rosettes with EAC3 but not with sheep erythrocytes. Four of the six LCL were principally surface IgD bearing and did not synthesize detectable Ig for secretion. Two of the cell lines were indistinguishable from cell lines from normal individuals: they had surface IgG, A, M, and D and synthesized and secreted Ig.