Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers
Fluorescence nanoscopy with optical sectioning by two-photon induced molecular switching using continuous-wave lasers
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DOI：
10.1002/cphc.200700655
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发表时间：
2008-02-01
期刊：
影响因子：
2.9
通讯作者：
Hell, Stefan W.
中科院分区：
文献类型：
--
作者：
Folling, Joenas;Belov, Vladimir;Hell, Stefan W.
在失去的十年中，场荧光显微镜方法已经发展到具有低于光波长的分辨率。为了超越衍射的限制作用，所有这些方法都以这样或那样的方式转换分子发射荧光的能力。在这里，我们提出了一种新的罗丹明酰胺，可以从一个非荧光到荧光状态的吸收一个或两个光子从连续波损失光束的光开关。这种明亮的标记物能够严格控制开/关切换，并在焦平面内提供低至15 nm的单分子定位精度。双光子诱导的非线性光开关的这种标记与连续波照明提供光学切片与简单的失败者设备。类似化合物的未来合成为具有成本效益的非侵入性光学切片荧光纳米显微镜带来了巨大的希望。
During the lost decade for-field fluorescence microscopy methods have evolved that have resolution for below the wavelength of light. To outperform the limiting role of diffraction, all these methods, in one way or another, switch the ability of a molecule to emit fluorescence. Here we present a novel rhodamine amide that can be photoswitched from a nonfluorescent to a fluorescent state by absorption of one or two photons from a continuous-wave loser beam. This bright marker enables strict control of on/off switching and provides single-molecule localization precision down to 15 nm in the focal plane. Two-photon induced nonlinear photoswitching of this marker with continuous-wave illumination offers optical sectioning with simple loser equipment. Future synthesis of similar compounds holds great promise for cost-effective fluorescence nanoscopy with noninvasive optical sectioning.