Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.
Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.
复制标题
目标特异性捕获提高了细菌病原体电化学检测的灵敏度。
DOI：
10.1128/jcm.01261-11
复制
发表时间：
2011
影响因子：
9.4
通讯作者：
Haake,DavidA
中科院分区：
文献类型：
--
作者：
Patel,Mayank;Gonzalez,Rodrigo;Halford,Colin;Lewinski,MichaelA;Landaw,ElliotM;Churchill,BernardM;Haake,DavidA
We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.
