A satisfactory technique for storing and staining malaria and other parasites in old blood films
A satisfactory technique for storing and staining malaria and other parasites in old blood films
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DOI：
10.1016/0035-9203(60)90086-9
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发表时间：
1960-01-01
期刊：
影响因子：
--
通讯作者：
MARYON, M.
中科院分区：
文献类型：
--
作者：
SHUTE, P. G.;MARYON, M.
未染色和未固定的厚血膜或薄血膜，如果储存在干燥器中并持续冷藏，即使经过长达3年的时间，也可以令人满意地染色。选择的染色剂是Giemsa盐水（1-10）。水性姬姆萨、利什曼或赖特染色剂不能给出一致令人满意的结果。暴露在户外的胶片很快就失去了任何可用的染色能力，这可能是由于它们溶血能力的丧失。|| 摘要：A. C.沃尔顿
Unstained and unfixed thick or thin blood films may be satisfactorily stained even after a lapse of as much as 3 years provided they have been stored in a desiccator and kept under constant refrigeration. The stain of choice is a Giemsa saline (1-10). Aqueous Giemsa, Leishman's or Wright's stains do not give as consistently satisfactory results. Films exposed to the open air rapidly lose their ability to take any sort of a usable stain, probably due to the loss of their ability to haemolyze. || ABSTRACT AUTHORS: A. C. Walton