Response of antioxidant enzymes to transfer from elevated carbon dioxide to air and ozone fumigation, in the leaves and roots of wild-type and a catalase-deficient mutant of barley
Response of antioxidant enzymes to transfer from elevated carbon dioxide to air and ozone fumigation, in the leaves and roots of wild-type and a catalase-deficient mutant of barley
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DOI：
10.1034/j.1399-3054.1998.1040217.x
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发表时间：
1998-10-01
影响因子：
6.4
通讯作者：
Lea, PJ
中科院分区：
文献类型：
--
作者：
Azevedo, RA;Alas, RM;Lea, PJ
过氧化氢酶缺陷突变体 (RPr 794) 和野生型 (cv. Maris Mink) 大麦 (Hordeum vulgare L.) 对应物。在高CO2 (0.7%) 中生长3 周，然后转移到空气和臭氧(120 nl l(-1)) 中，在光和荫下培养3 天。分析叶和根的过氧化氢酶（CAT，EC 1.11.1.6）、超氧化物歧化酶（SOD。EC 1.15.1.1）和谷胱甘肽还原酶（GR，EC 1.6.4.2）活性。 RPr 79/4 过氧化氢酶缺陷突变体叶子中的 CAT 活性约为 Maris Mink 中测定的 5±10%。但在根部，两种基因型的活性水平大致相同。将植物从 0.7% CO2 转移到空气或臭氧后，Maris Mink 叶子中的 CAT 活性增加，最高达到 5 倍。在阴凉处和臭氧下放置 4 天后。对于过氧化氢酶缺陷突变体。在光/空气和光/臭氧处理中仅观察到 CAT 活性的小幅增加。在根部。植物从 0.7% CO2 转移后，两种基因型的 CAT 活性一致下降。从 0.7% CO2 转移后，两种基因型的叶和根中的总可溶性 SOD 活性均增加。在非变性 PAGE 后对从叶子中分离出的 SOD 进行分析，发现存在多达八种 SOD 同工酶，分为 Mn-SOD 或 Cu/Zn-SOD：未检测到 Fr-SOD。观察到 Mn- 和 Cu/Zn-SOD 同工酶的显着变化；然而，他们无法解释 SOD 总活性的增加。病叶。从 0.7% CO2 转移后，Maris Mink 和 RPr 79/4 中的 GR 活性也有所增加：然而，无法建立恒定的模式，而在根中，GR 活性在处理 4 天后降低。数据表明，升高的 CO2 降低了大麦叶片的氧化应激，并且在植物从升高的 CO2 转移后，可溶性 CAT 和 SOD 活性迅速增加。与处理方式无关（光、阴影、空气或臭氧）。
A catalase-deficient mutant (RPr 794) and the wild-type (cv. Maris Mink) barley (Hordeum vulgare L.) counterpart. were grown for 3 weeks in high CO2 (0.7%) and then transferred to air and ozone (120 nl l(-1)) in the light and shade for a period of 3 days. Leaves and roots were analysed for catalase (CAT, EC 1.11.1.6), superoxide dismutase (SOD. EC 1.15.1.1) and glutathione reductase (GR, EC 1.6.4.2) activities. CAT activity in the leaves of the RPr 79/4 catalase-deficient mutant was around 5 10% of that determined in Maris Mink. but in the roots, both genotypes contained approximately the same levels of activity. CAT activity in Maris Mink increased in the leaves after transferring plants from 0.7% CO2 to air or ozone, reaching a maximum of 5-fold. after 4 days in shade and ozone. For the catalase-deficient mutant. only small increases in CAT activity were observed in light/air and light/ozone treatments. In the roots. CAT activity decreased consistently in both genotypes, after plants were transferred from 0.7% CO2.The total soluble SOD activity in the leaves and roots of both genotypes increased after plants were transferred from 0.7% CO2. The analysis of SOD isolated from leaves following non-denaturing PAGE, revealed the presence of up to eight SOD isoenzymes classified as Mn-SOD or Cu/Zn-SODs: Fr-SOD was not detected. Significant changes in Mn- and Cu/Zn-SOD isoenzymes were observed; however, they could not account for the increase in total SOD activity. Ill leaves. GR activity also increased in Maris Mink and RPr 79/4, following transfer from 0.7% CO2: however, no constant pattern could be established, while in roots, GR activity was reduced after 4 days of the treatments.The data suggest that elevated CO2 decreases oxidative stress in barley leaves and that soluble CAT and SOD activities increased rapidly after plants were transferred from elevated CO2. irrespective of the treatment (light, shade, air or ozone).