Fetal Thymus Organ Culture for T Cell Development Studies
Fetal Thymus Organ Culture for T Cell Development Studies
复制标题
用于 T 细胞发育研究的胎儿胸腺器官培养
DOI：
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发表时间：
1992
期刊：
影响因子：
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通讯作者：
F. Ramsdell
中科院分区：
文献类型：
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作者：
F. Ramsdell
大多数T细胞发育发生在胸腺内，包括一系列的选择过程，在很大程度上仍然知之甚少。通过描述T细胞的多个表型亚群及其成熟关系，T细胞发育的研究已经大大推进。本单元描述了一个系统，通过培养整个小鼠胎儿胸腺叶，观察和调节T细胞体外发育。基本方案描述了胎儿胸腺的分离和用于允许在transwell板上正常T细胞发育的培养系统。它比第一个替代方案更容易实施，并且稍微更昂贵，第一个替代方案描述了在Gelfoam海绵上培养的更传统的方法（最初用于开发培养系统）。在第二个替代方案中描述了使用2′-脱氧鸟苷从胸腺叶中去除造血细胞并随后用前体细胞重建的方法。该方案对耐受性和T细胞选择的研究有价值。在支持方案中，概述了改变和监测T细胞发育的方法。
Most T cell development occurs within the thymus and includes a series of selection processes that are, in large part, still poorly understood. Studies of T cell development have been greatly advanced by the description of multiple phenotypic subsets of T cells and their maturational relationships. This unit describes a system for observing and modulating T cell development in vitro via the culture of entire mouse fetal thymic lobes. The basic protocol delineates the isolation of fetal thymi and the culture system used to allow for normal T cell development on transwell plates. It is easier and somewhat more expensive to carry out than the first alternate protocol which describes a more traditional method of culture on Gelfoam sponges (used originally to develop the culture system). A method for depleting hematopoietic cells from thymic lobes using 2′‐deoxyguanosine and subsequent reconstitution with precursor cells is described in the second alternate protocol . This protocol is valuable for the study of tolerance and T cell selection. In the support protocol , methods of altering and monitoring T cell development are outlined.