INDUCTION OF ANTERIOR-CHAMBER ASSOCIATED IMMUNE DEVIATION IN RATS RECEIVING INTRACAMERAL INJECTIONS OF RETINAL S-ANTIGEN
INDUCTION OF ANTERIOR-CHAMBER ASSOCIATED IMMUNE DEVIATION IN RATS RECEIVING INTRACAMERAL INJECTIONS OF RETINAL S-ANTIGEN
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DOI：
10.3109/02713688809031820
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发表时间：
1988-06-01
影响因子：
2
通讯作者：
STREILEIN, JW
中科院分区：
文献类型：
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作者：
MIZUNO, K;CLARK, AF;STREILEIN, JW
将多种抗原（包括移植抗原、半抗原和病毒糖蛋白）注射到小鼠眼睛的前房，会产生一种典型的免疫反应谱，其中抑制细胞介导的免疫占主导地位。这种现象被称为前房相关免疫偏差（ACAID）。最近发现可溶性抗原也能诱导小鼠ACAID，我们研究了一种重要的可溶性眼部抗原，视网膜S抗原（S Ag）可能能够诱导大鼠ACAID的可能性。我们发现，将S Ag与佐剂一起注射到成年Lewis大鼠的眼睛前房，既不会引起可检测到的体液免疫反应，也不会引起细胞介导的免疫反应。然而，以这种方式在腹腔内注射S Ag的动物，通过产生血清抗S Ag抗体，对随后注射到后足垫的CFA中S Ag的免疫原性剂量有反应。重要的是，这些大鼠没有表现出抗原特异性延迟超敏反应。此外，前房预处理动物的淋巴样细胞在过继转移时，抑制了初次受体的S ag特异性延迟超敏反应的发展，这表明一种主动抑制机制已经产生。因此，在成年Lewis大鼠体内注射一种可溶性抗原可产生ACAID。讨论了ACAID可能与S - Ag诱导的葡萄膜炎的发展有关的可能性。
The injection of a variety of antigens, including transplantation antigens, haptens, and viral glycoproteins, into the anterior chamber of eyes of mice produces a characteristic spectrum of immune responses in which suppressed cell mediated immunity dominates. This phenomenon is called Anterior Chamber Associated Immune Deviation (ACAID). Having recently demonstrated that soluble antigens also can induce ACAID in mice, we examined the possibility that an important soluble ocular antigen, retinal S antigen (S Ag) may be capable of inducing ACAID in rats. We have found that injection of S Ag with adjuvant into the anterior chamber of eyes of adult Lewis rats evokes neither a humoral nor a cell mediated immune response that can be detected. However, animals that received S Ag intracamerally in this manner did respond to a subsequent immunogenic dose of S Ag in CFA injected into the hind foot pads, by producing serum anti S Ag antibodies. Importantly, these rats failed to display antigen-specific delayed hypersensitivity. Moreover, lymphoid cells from anterior chamber pre-treated animals, when adoptively transferred, inhibited the development of S Ag-specific delayed hypersensitivity in naive recipients indicating that an active suppression mechanism had been generated. Thus, a soluble antigen injected intracamerally in adult Lewis rats can generate ACAID. The possibility that ACAID may be relevant to the development of S Ag induced uveitis is discussed.