Solid-State Nanopore Detection of Protein Complexes: Applications in Healthcare and Protein Kinetics
Solid-State Nanopore Detection of Protein Complexes: Applications in Healthcare and Protein Kinetics
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DOI：
10.1002/smll.201201423
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发表时间：
2013-03-11
期刊：
影响因子：
13.3
通讯作者：
Kim, Min Jun
中科院分区：
文献类型：
--
作者：
Freedman, Kevin J.;Bastian, Arangassery R.;Kim, Min Jun
蛋白质缀合提供了对许多生物现象的独特观察，并已用于分子识别目的数十年。在这项研究中，使用固态纳米孔检测gp120相关的复合物进行了研究。它们表现出与抗gp120抗体单体和二聚体的单价和多价结合。为了研究与纳米孔相关的许多实际应用的可行性，尝试在异质蛋白质样品内检测特定蛋白质复合物，并研究电压对复合蛋白质的作用。据发现，孔内的电场可以导致在实验测量的瞬态事件持续时间内的自由易位的蛋白质复合物的解结合。解结合时间与电压的强依赖性可用于通过增加可探测的额外特异性水平来改善纳米孔系统的检测能力。这些数据为未来的蛋白质特异性检测方案提供了强有力的框架，这些方案已被证明在真实世界的样本和检测事件的自动多维方法领域是可行的。
Protein conjugation provides a unique look into many biological phenomena and has been used for decades for molecular recognition purposes. In this study, the use of solid-state nanopores for the detection of gp120-associated complexes are investigated. They exhibit monovalent and multivalent binding to anti-gp120 antibody monomer and dimers. In order to investigate the feasibility of many practical applications related to nanopores, detection of specific protein complexes is attempted within a heterogeneous protein sample, and the role of voltage on complexed proteins is researched. It is found that the electric field within the pore can result in unbinding of a freely translocating protein complex within the transient event durations measured experimentally. The strong dependence of the unbinding time with voltage can be used to improve the detection capability of the nanopore system by adding an additional level of specificity that can be probed. These data provide a strong framework for future protein-specific detection schemes, which are shown to be feasible in the realm of a real-world' sample and an automated multidimensional method of detecting events.