Evaluation of immunomodulatory chemicals: alteration of macrophage function in vitro.
Evaluation of immunomodulatory chemicals: alteration of macrophage function in vitro.
复制标题
免疫调节化学物质的评估：体外巨噬细胞功能的改变。
DOI：
10.1016/0041-008x(84)90041-3
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发表时间：
1984
影响因子：
3.8
通讯作者：
R. Hinsdill
中科院分区：
文献类型：
--
作者：
P. E. Tam;R. Hinsdill
为了建立一种有效的检测巨噬细胞功能潜在调节剂的筛选方法，测试了25种化合物对小鼠腹膜巨噬细胞(PM)非特异性吞噬和杀灭酿酒酵母的能力。平行研究监测PM的活性，以区分吞噬功能的有效调节和仅由细胞毒性引起的变化。葡聚糖硫酸盐、ι-卡拉胶、λ-卡拉胶、二丙基二氯化锡和二正辛基二氯化锡在未产生明显毒性的浓度下，显著降低了能够摄取酵母的PM的百分比。卡拉胶还减少了每PM摄入的酵母的平均数量，同时增加了PM杀死酵母的能力。消炎痛和硫酸葡聚糖对PM的杀菌活性有抑制作用。二正辛基二氯化锡和葡聚糖硫酸盐降低了膜的粘附性，而左旋咪唑则增强了膜的粘附性。香草醛、没食子酸丙酯、醋酸铅、氢化可的松21-磷酸和氢化可的松21-丁二酸氢化可的松降低了PM能够摄取酵母的百分比，但不低于对照的50%。没食子酸、对羟基苯甲酸甲酯、氯化汞、氯化镉、氯化镍、氯化铬、二甲基二氯化锡、二乙基二氯化锡、二丁基二氯化锡、四正辛基锡、赤霉酸、环磷酰胺和硫唑嘌呤在非细胞毒性剂量下不改变PM的吞噬功能。结果表明，化学物质可以分为三大类：无效的、弱的和有效的PM功能调节剂。
In an effort to develop a useful screening procedure for detecting potential modulators of macrophage function, 25 chemicals were tested for their ability to alter the nonspecific phagocytosis and killing of Saccharomyces cerevisiae by elicited mouse peritoneal macrophages (PM). Parallel studies monitored PM viability so as to distinguish between effective modulation of phagocytic function and changes due solely to cytotoxicity. Dextran sulfate, ι-carrageenan, λ-carrageenan, dipropyltin dichloride, and di-n-octyltin dichloride, at concentrations which did not produce overt toxicity, significantly reduced the percentage of PM capable of ingesting yeast. The carrageenans also decreased the average number of yeast ingested per PM, while increasing the ability of PM to kill yeast. Microbicidal activity was suppressed in PM treated with indomethacin and dextran sulfate. Di-n-octyltin dichloride and dextran sulfate diminished the adherence of PM, whereas levamisole enhanced PM adherence. Vanillin, propyl gallate, lead acetate, hydrocortisone 21-phosphate, and hydrocortisone 21-hemisuccinate decreased the percentage of PM capable of ingesting yeast, but not below 50% of the control. Gallic acid, methyl paraben, mercuric chloride, cadmium chloride, nickel chloride, chromic chloride, dimethyltin dichloride, diethyltin dichloride, dibutyltin dichloride, tetra-n-octyltin, gibberellic acid, cyclophosphamide, and azathioprine did not alter PM phagocytic function at noncytotoxic doses. The results indicate that chemicals can be grouped into three broad categories as either ineffective, weak modulators, or effective modulators of PM function.
使用体外抗体产生测定法评估化学品的免疫调节作用。
影响因子：
8.3
作者：
Kutz,SA;Hinsdill,RD;Weltman,DJ
通讯作者：
Weltman,DJ