Follicle-stimulating hormone regulates glycolysis of water buffalo follicular granulosa cells through AMPK/SIRT1 signalling pathway
Follicle-stimulating hormone regulates glycolysis of water buffalo follicular granulosa cells through AMPK/SIRT1 signalling pathway
复制标题
卵泡刺激素通过AMPK/SIRT1信号通路调节水牛卵泡颗粒细胞的糖酵解
DOI：
10.1111/rda.14039
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发表时间：
2021-11-11
影响因子：
1.7
通讯作者：
Deng, Yanfei
中科院分区：
文献类型：
--
作者：
Pan, Yu;Zhu, Jianzong;Deng, Yanfei
卵泡颗粒细胞（follicular granlosa cells, GCs）的糖酵解是卵母细胞能量代谢底物的主要来源，与哺乳动物卵泡发育密切相关。卵泡刺激素（follicle-stimulating hormone， FSH）调节着GCs的许多生理功能。相比之下，FSH是否调节GCs的糖酵解及其机制尚不清楚。本研究探讨了不同直径水牛卵泡GCs的FSH浓度与糖酵解水平的相关性，并进一步探讨了FSH对体外培养GCs糖酵解的调节机制。结果显示，卵泡液乳酸浓度及糖酵解相关基因表达水平的变化趋势与不同直径卵泡卵泡液FSH浓度的变化趋势一致。体外FSH处理GCs时，糖酵解相关基因、乳酸生成和葡萄糖摄取的表达水平相应升高（p < 0.05）。此外，我们发现AMPK/Sirtuin1 （SIRT1）通路相关基因在GCs中的表达趋势与糖酵解相关基因的表达趋势一致，且与体内或体外培养FSH浓度呈正相关。SIRT1的激活增加了GCs中糖酵解关键蛋白的表达水平和乳酸的产生，而SIRT1的抑制则表现出相反的效果。总的来说，体内或体外培养的水牛GCs的糖酵解与FSH浓度呈正相关。AMPK/SIRT1通路在FSH对GCs糖酵解的调控中起重要作用。我们的发现将丰富对卵泡刺激素调节水牛卵泡发育的认识。
Glycolysis in follicular granulosa cells (GCs) is the primary source of energy metabolism substrate of oocytes and is closely related to follicular development in mammals. Many physiological functions of GCs are regulated by follicle-stimulating hormone (FSH). In contrast, whether FSH regulates the glycolysis of GCs and its mechanism remains unclear. This study explored the correlation between FSH concentration and glycolysis level of GCs from different diameters of water buffalo follicles, and further explored the mechanism of FSH regulation in glycolysis in vitro cultured GCs. Results showed the variation trend of lactic acid concentration in follicular fluid and the expression level of glycolysis-related genes in GCs were consistent with the variation trend of FSH concentration in follicular fluid from follicles with different diameters. When GCs were treated with FSH in vitro, the expression level of glycolysis-related genes, lactate production and glucose uptake increased correspondingly (p < .05). Furthermore, we found that expression trend of AMPK/Sirtuin1 (SIRT1) pathway-related genes in GCs was consistent with the expression trend of glycolysis-related genes and was positively correlated with FSH concentrations in vivo or cultured in vitro. Activation of SIRT1 increased the expression level of glycolytic key proteins and lactic acid production in GCs, while inhibition of SIRT1 showed the opposite effect. In general, glycolysis in water buffalo GCs in vivo or cultured in vitro was positively correlated with FSH concentration. AMPK/SIRT1 pathway plays an important role in the regulation of FSH on glycolysis in GCs. Our findings will enrich the understanding of FSH regulating the development of water buffalo follicles.