Possible involvement of Wnt11 in colorectal cancer progression
Possible involvement of Wnt11 in colorectal cancer progression
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DOI：
10.1002/mc.21845
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发表时间：
2013-03-01
影响因子：
4.6
通讯作者：
Hinoda, Yuji
中科院分区：
文献类型：
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作者：
Nishioka, Mitsuaki;Ueno, Koji;Hinoda, Yuji
我们的前期研究揭示了Frizzled-7（FZD 7）在结直肠癌（CRC）中的表达及其在CRC进展中的可能作用。在这项研究中，我们通过定量RT-PCR测量了候选FZD 7配体Wnt 3和Wnt 11在结肠癌细胞系（n=7）和原发性CRC组织（n=133）中的表达水平。我们还检查了Wnt 11的功能效果与使用Wnt 11转染结肠癌HCT-116细胞。Wnt 11转染细胞的增殖和迁移/侵袭活性均高于对照细胞。Western blot分析显示，转染Wnt 11后JNK和c-jun的磷酸化水平增加。Wnt 11 mRNA在I、II、III或IV期肿瘤组织中的表达显著高于非肿瘤组织（总体P
Our previous report revealed that the expression of Frizzled-7 (FZD7) in colorectal cancer (CRC) and its possible role in CRC progression. In this study we measured the expression levels of candidate FZD7 ligands, Wnt3 and Wnt11 in colon cancer cell lines (n=7) and primary CRC tissues (n=133) by quantitative RT-PCR. We also examined the functional effects of Wnt11 with the use of Wnt11 transfectants of colon cancer HCT-116 cells. Wnt11 transfectants showed the increased proliferation and migration/invasion activities compared to mock cells. Western blot analysis of transfectants revealed that phosphorylation of JNK and c-jun was increased after Wnt11 transfection. Wnt11 mRNA expression was significantly higher in the stage I, II, III, or IV tumor tissues than in non-tumor tissues (overall P