PRIMARY STRUCTURE OF THE PRECURSOR TO THE 3 MAJOR SURFACE-ANTIGENS OF PLASMODIUM-FALCIPARUM MEROZOITES
PRIMARY STRUCTURE OF THE PRECURSOR TO THE 3 MAJOR SURFACE-ANTIGENS OF PLASMODIUM-FALCIPARUM MEROZOITES
复制标题
DOI：
10.1038/317270a0
复制
发表时间：
1985-01-01
期刊：
影响因子：
64.8
通讯作者：
FREEMAN, RR
中科院分区：
文献类型：
--
作者：
HOLDER, AA;LOCKYER, MJ;FREEMAN, RR
最近，已经鉴定了一类高相对分子质量（Mr）的蛋白质抗原，其可以诱导针对血液期疟疾的保护性免疫1 -5。在恶性疟原虫中，该蛋白质的Mr为195，000（P195）6，7。它是Mr 83，000（83 K）、42 K和19 K三种蛋白质的前体，这三种蛋白质是裂殖子的主要表面抗原8，9;因此，它也可用于针对P的免疫。恶性疟原虫。三项研究描述了P195基因序列部分的单个短互补DNA克隆的分离，这三项研究已被证实4，10，18。在这里，我们描述了P195基因的完整结构，确定从进一步的DNA克隆，其组织内的基因组DNA和特定的加工片段内的主要氨基酸序列的位置。
Recently, a class of protein antigens of high relative molecular mass (Mr) which can induce protective immunity against blood-stage malaria has been identified1–5. InPlasmodium falciparumthe protein has aMrof ∼195,000 (P195)6,7. It is the precursor of three proteins ofMr83,000 (83K), 42K and 19K which are the major surface antigens of merozoites8,9; thus it may also be useful for immunization againstP. falciparum. Three studies describing the isolation of single short complementary DNA clones for part of the P195 gene sequence have been reported4,10,18. Here we describe the complete structure of the P195 gene determined from further DNA clones, its organization within genomic DNA and the location of the specific processing fragments within the primary amino-acid sequence.