α-Tocopherols from the Formosan Soft Coral Lobophytum crassum
α-Tocopherols from the Formosan Soft Coral Lobophytum crassum
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DOI：
10.1246/bcsj.20110051
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发表时间：
2011-07-15
影响因子：
4
通讯作者：
Duh, Chang-Yih
中科院分区：
文献类型：
--
作者：
Cheng, Shi-Yie;Lin, Shih-Tseng;Duh, Chang-Yih
在我们筛选海洋生物的生物活性代谢产物中，我们从台湾软珊瑚Lobophytum crassum中获得了两种新的α-生育酚，命名为crystaltocopherols A（1）和B（2）。化合物1和2的结构经核磁共振和高分辨电喷雾质谱分析确定。本文研究了1、2、天然维生素E和合成维生素E对肿瘤细胞的细胞毒作用和对人巨细胞病毒的抗病毒作用。化合物1和2对P-388（小鼠淋巴细胞白血病）细胞系表现出中等的细胞毒性，IC 50分别为3.2和2.7 μ g mL（-1）。此外，化合物2对HT-29（人结肠腺癌）也显示出中等的细胞毒性，IC 50为3.9 μ g/mL（-1）。然而，天然维生素E和合成维生素E对选定的癌细胞系没有细胞毒性，并且在1 μ g/mL（-1）的浓度下，对人巨细胞病毒的抗病毒活性抑制结果均为阴性。
In our screening of marine organisms for bioactive metabolites we have obtained two new alpha-tocopherols, designated as crassumtocopherols A (1) and B (2), from the Formosan soft coral Lobophytum crassum. The structures of 1 and 2 were determined on the basis of comprehensive NMR and HR-ESI-MS analyses. The cytotoxicity of 1, 2, natural vitamin E, and synthetic vitamin E against cancer cell lines and antiviral activity against human cytomegalovirus were evaluated in vitro. Compounds 1 and 2 exhibited moderate cytotoxicity against P-388 (mouse lymphocytic leukemia) cell lines with IC50 of 3.2 and 2.7 mu g mL(-1), respectively. In addition, compound 2 also displayed moderate cytotoxicity against HT-29 (human colon adenocarcinoma) with an IC50 of 3.9 mu g mL(-1). However, natural vitamin E and synthetic vitamin E were not cytotoxic to the selected cancer cell lines and the results for inhibition of antiviral activity against human cytomegalovirus are all negative at a concentration of 1 mu g mL(-1).