miRNA Regulation Network Analysis in Qianliening Capsule Treatment of Benign Prostatic Hyperplasia.
miRNA Regulation Network Analysis in Qianliening Capsule Treatment of Benign Prostatic Hyperplasia.
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前列宁胶囊治疗良性前列腺增生的miRNA调控网络分析
DOI：
10.1155/2015/365484
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Hong Z
中科院分区：
文献类型：
--
作者：
Liu L;Wan Y;Shen A;Zhao J;Lin J;Zhong X;Zhang Y;Hong Z
客观的。本研究的目的是评估前列宁胶囊（QC）治疗良性前列腺增生（BPH）的分子机制。方法。分别用0、1.25、2.5和5mg/mL QC处理良性前列腺增生上皮细胞系BPH-1 48小时。分析细胞活力的评价以及BPH-1细胞基因表达和miRNA表达谱的形态变化观察。使用实时定量PCR来确认miRNA和基因表达的变化。基于 GO 和 KEGG 通路的方法用于研究受差异表达 mRNA 影响的生物学功能和信号通路。结果。 QC 抑制 BPH-1 细胞增殖。与未处理的对照细胞相比，在 QC 处理的 BPH-1 细胞中观察到 19 种上调和 2 种下调的 miRNA 表达差异。两组之间鉴定出 107 个上调基因和 71 个下调基因。基于mRNA失调的信号通路显着丰富，主要涉及细胞增殖、凋亡等的调节。此外，miRNA-mRNA 网络分析通过概述 miRNA 和相关基因的相互作用来整合这些 miRNA 和基因。结论。该研究首次报道了 QC 处理的 BPH-1 细胞中差异表达的 miRNA 和 mRNA。
Objective. The objective of this study was to evaluate the molecular mechanism by which Qianliening capsule (QC) treats benign prostatic hyperplasia (BPH). Methods. Benign prostatic hyperplasia epithelial cell line BPH-1 was treated with 0, 1.25, 2.5, and 5 mg/mL QC for 48 h, respectively. Evaluation of cell viability and observation of morphologic changes of BPH-1 cell gene expression and miRNA expression profiles were analyzed. Real-time quantitative PCR was used to confirm changes in miRNA and gene expression. GO and KEGG pathway-based approaches were used to investigate biological functions and signaling pathways affected by differentially expressed mRNAs. Results. QC inhibited BPH-1 cell proliferation. Differential expression of 19 upregulated and 2 downregulated miRNAs was observed in QC-treated BPH-1 cells compared to untreated control cells. 107 upregulated and 71 downregulated genes were identified between the two groups. Significantly enriched signaling pathways based on deregulated mRNAs were mainly involved in regulation of cell proliferation, apoptosis, and so on. Additionally, miRNA-mRNA network analysis integrated these miRNAs and genes by outlining interactions of miRNA and related genes. Conclusion. The study was the first report of differentially expressed miRNA and mRNA in QC-treated BPH-1 cells.