DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy.
DoG Picker and TiltPicker: software tools to facilitate particle selection in single particle electron microscopy.
复制标题
狗拾取器和倾斜机：促进单个颗粒电子显微镜中颗粒选择的软件工具。
DOI：
10.1016/j.jsb.2009.01.004
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发表时间：
2009-05
影响因子：
3
通讯作者：
Carragher, B.
中科院分区：
文献类型：
--
作者：
Voss, N. R.;Yoshioka, C. K.;Radermacher, M.;Potter, C. S.;Carragher, B.
利用透射电子显微镜分析大分子复合物的结构是一项艰巨的任务。这个过程中的许多步骤都强烈依赖于预先存在的结构知识的帮助，当这些信息不可用时，就会变得非常复杂。在这里，我们提出了两个软件工具，旨在促进颗粒拾取，在未知大分子的单颗粒处理的早期阶段。第一个工具DoG Picker是一种基于高斯差分（DoG）图像变换的高效且合理通用的粒子拾取器。它可以单独运行，作为一个无参考的粒子拾取器，具有基于大小对粒子进行排序的独特能力，或者它也可以作为一种方法来引导模板的创建或其他粒子拾取器的训练数据集。第二个工具是TiltPicker，一个交互式图形界面应用程序，旨在简化从倾斜对数据集中选择粒子对的过程。在许多方面，TiltPicker是SPIDER WEB倾斜粒子拾取器的重新实现，但构建在现代计算机框架上，使其更易于部署和维护。TiltPicker程序还包括几个有用的新功能，超出了它的前身。
Solving the structure of macromolecular complexes using transmission electron microscopy can be an arduous task. Many of the steps in this process rely strongly on the aid of pre-existing structural knowledge, and are greatly complicated when this information is unavailable. Here we present two software tools meant to facilitate particle picking, an early stage in the single-particle processing of unknown macromolecules. The first tool, DoG Picker, is an efficient and reasonably general, particle picker based on the Difference of Gaussians (DoG) image transform. It can function alone, as a reference-free particle picker with the unique ability to sort particles based on size, or it can also be used as a way to bootstrap the creation of templates or training datasets for other particle pickers. The second tool is TiltPicker, an interactive graphical interface application designed to streamline the selection of particle pairs from tilted-pair datasets. In many respects, TiltPicker is a re-implementation of the SPIDER WEB tilted-particle picker, but built on modern computer frameworks making it easier to deploy and maintain. The TiltPicker program also includes several useful new features beyond those of its predecessor.
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