SlJAZ10 and SlJAZ11 mediate dark-induced leaf senescence and regeneration.
SlJAZ10 and SlJAZ11 mediate dark-induced leaf senescence and regeneration.
复制标题
SlJAZ10 和 SlJAZ11 介导暗诱导的叶片衰老和再生
DOI：
10.1371/journal.pgen.1010285
复制
发表时间：
2022-07
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
在进化适应过程中，植物的正常生长发育与环境胁迫之间建立起自我调节机制。植物激素茉莉酸（JA）是植物防御和发育的关键纽带，而茉莉酸-ZIM结构域（JAZ）阻遏蛋白是JA信号通路的关键组分。在这里，我们表明，JAZ的表达受到叶片衰老的转录组数据。进一步的研究表明，SlJAZ 10和SlJAZ 11正调控叶片衰老，SlJAZ 11还可以促进植株再生。此外，我们揭示了SlJAV 1-SlWRKY 51（JW）复合物可以在正常生长条件下抑制JA生物合成。在损伤后，SlJAZ 10和SlJAZ 11可以通过电信号和Ca 2+波的作用调节JW复合物的活性，这反过来又影响JA的生物合成，导致SlJAZ 10-OE和SlJAZ 11-OE转基因植物之间的再生表型的差异。此外，SlRbcs-3B还能维持SlJAZ 11蛋白的稳定性，防止其降解。SlJAZ 10和SlJAZ 11不仅共同作为JA信号转导的抑制子参与叶片衰老，而且还通过协同的电信号、Ca ~（2+）波、激素和转录调控来调控植物再生。我们的研究为SlJAZ 11诱导再生的机制提供了重要的见解。在植物中，衰老是发育的最后阶段，但再生可以帮助它们超越这一阶段。植株再生对于繁殖至关重要，并在栽培作物中尽可能保持优良性状。茉莉酸信号传导可以感知环境信号并整合各种调控机制，以确保植物再生在最佳条件下发生。本研究对JAZ-JAV 1-WRKY 51复合物进行了进一步优化，确定了SlJAZ 10和SlJAZ 11的功能是通过直接结合SlAOC启动子的W-box来促进SlJAV 1-SlWRKY 51复合物的抑制活性，从而负调控JA的生物合成。进一步的研究结果表明，S1 JAZ 10和S1 JAZ 11再生过程中电信号、Ca ~（2+）波、激素和转录调控的差异是导致再生的主要原因。此外，我们发现SlRbcs-3B可以保持SlJAZ 11的蛋白质稳定性，以保护其不被降解。总之，尽管SlJAZ 10和SlJAZ 11都可以起到衰老的作用，但只有SlJAZ 11具有重要的再生促进功能。
During evolutionary adaptation, the mechanisms for self-regulation are established between the normal growth and development of plants and environmental stress. The phytohormone jasmonate (JA) is a key tie of plant defence and development, and JASMONATE-ZIM DOMAIN (JAZ) repressor proteins are key components in JA signalling pathways. Here, we show that JAZ expression was affected by leaf senescence from the transcriptomic data. Further investigation revealed that SlJAZ10 and SlJAZ11 positively regulate leaf senescence and that SlJAZ11 can also promote plant regeneration. Moreover, we reveal that the SlJAV1-SlWRKY51 (JW) complex could suppress JA biosynthesis under normal growth conditions. Immediately after injury, SlJAZ10 and SlJAZ11 can regulate the activity of the JW complex through the effects of electrical signals and Ca2+ waves, which in turn affect JA biosynthesis, causing a difference in the regeneration phenotype between SlJAZ10-OE and SlJAZ11-OE transgenic plants. In addition, SlRbcs-3B could maintain the protein stability of SlJAZ11 to protect it from degradation. Together, SlJAZ10 and SlJAZ11 not only act as repressors of JA signalling to leaf senescence, but also regulate plant regeneration through coordinated electrical signals, Ca2+ waves, hormones and transcriptional regulation. Our study provides critical insights into the mechanisms by which SlJAZ11 can induce regeneration. In plants, senescence is the final stage of development, but regeneration can help them beyond the stage. Plants regeneration is essential for propagation, and in cultivated crops to maintain excellent traits as close as possible. JA signaling can sense environmental signals and integrate various regulatory mechanisms to ensure plants regeneration occurs under optimal conditions. In this work, the JAZ-JAV1-WRKY51 complexes with reported was further optimized, the function of SlJAZ10 and SlJAZ11 was identified to promote inhibitory activity of SlJAV1-SlWRKY51 complex which negatively regulated JA biosynthesis by direct binding of the W-box of the SlAOC promoter. The results of further investigation suggest that the differences in regulation of electrical signals, Ca2+ waves, hormones and transcriptional regulation are responsible for the regeneration between SlJAZ10 and SlJAZ11. In addition, we have found that SlRbcs-3B could maintain the protein stability of SlJAZ11 to protect it from degradation. In summary, despite both SlJAZ10 and SlJAZ11 can function as senescence, only SlJAZ11 has an important promoting function for regeneration.