Alpha 1-adrenergic stimulation of rat myocardial cells increases protein synthesis.
Alpha 1-adrenergic stimulation of rat myocardial cells increases protein synthesis.
复制标题
大鼠心肌细胞的α1-肾上腺素能刺激增加蛋白质合成。
DOI：
10.1152/ajpheart.1986.251.5.h1076
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Chien,KR
中科院分区：
文献类型：
--
作者：
Meidell,RS;Sen,A;Henderson,SA;Slahetka,MF;Chien,KR
在新生大鼠心脏细胞的原代培养中，研究了肾上腺素能刺激对蛋白质合成、降解和积累速率的影响。用去甲肾上腺素处理心肌细胞可增加总细胞蛋白含量和放射性标记酪氨酸掺入三氯乙酸不溶性蛋白的速率。α 1-肾上腺素能阻滞剂，而不是α 2-或β-肾上腺素能阻滞剂，抑制这些去甲肾上腺素诱导的增加。去甲肾上腺素刺激的平衡标记的动力学和从组合的平衡和脉冲标记的蛋白质合成的速率增加，而蛋白质降解估计释放先前纳入放射性标记的酪氨酸或脉冲追逐实验不受影响。为了确定α 1-肾上腺素能刺激是否产生类似的影响，对肌原纤维蛋白的营业额，合成和降解率进行了估计的肌原纤维富集的蛋白质组分和肌球蛋白重链和肌动蛋白。去甲肾上腺素治疗产生的肌原纤维蛋白的合成增加，而没有显着改变降解速率。这些实验表明，α 1-肾上腺素能刺激通过加速蛋白质合成增加心肌细胞蛋白质含量。
The effects of adrenergic stimulation on the rates of protein synthesis, degradation, and accumulation were examined in primary cultures of neonatal rat heart cells. Treatment of myocardial cells with norepinephrine increased total cellular protein content and the rate of incorporation of radiolabeled tyrosine into trichloroacetic acid insoluble protein. alpha 1-Adrenergic, but not alpha 2- or beta-adrenergic blockade, inhibited these norepinephrine induced increases. The rate of protein synthesis estimated from the kinetics of equilibrium labeling and from combined equilibrium and pulse labeling was increased by norepinephrine stimulation, whereas protein degradation estimated by release of previously incorporated radiolabeled tyrosine or in pulse-chase experiments was unaffected. To determine whether alpha 1-adrenergic stimulation produced similar effects on the turnover of myofibrillar proteins, rates of synthesis and degradation were estimated for a myofibrillar-enriched protein fraction and for myosin heavy chain and actin. Norepinephrine treatment produced increases in the synthesis of myofibrillar protein without significantly altering degradation rates. These experiments suggest that alpha 1-adrenergic stimulation increases myocardial cell protein content by accelerating protein synthesis.