Spectroscopic studies on the inhibitory effects of ionic liquids on lipase activity
Spectroscopic studies on the inhibitory effects of ionic liquids on lipase activity
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离子液体对脂肪酶活性抑制作用的光谱研究
DOI：
10.1016/j.saa.2016.01.047
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发表时间：
2016-04-15
影响因子：
4.4
通讯作者：
Li, Yan
中科院分区：
文献类型：
--
作者：
Fan, Yunchang;Dong, Xing;Li, Yan
利用紫外-可见光谱研究了离子液体对脂肪酶活性的影响，并利用荧光技术从分子水平探讨了离子液体与脂肪酶的作用机理。实验结果表明，离子液体对脂肪酶有抑制作用，且抑制程度与离子液体的化学结构密切相关。Cl-和Br-基离子液体的抑制能力随着离子液体阳离子中烷基链长度的增加而增加。通过分析脂肪酶在离子液体中的荧光行为，得到了热力学参数、焓变和熵变。Δ H和Δ S均为正，表明疏水性是Cl-和Br-基离子液体的主要驱动力。对于BF_4-、CF_3SO_3-、ClO_4-和N（CN）（2）（-）-离子液体，氢键是主要的驱动力。为了更全面地理解离子液体对脂肪酶活性的影响，必须同时考虑疏水性和氢键的作用。建立了离子液体的疏水性和氢键结合能力与抑制能力的回归方程。(C)© 2016 Elsevier B. V.版权所有。
The effects of ionic liquids (ILs) on the lipase activity were studied by UV-Vis spectroscopy and the IL-lipase interaction mechanism at the molecular level was investigated by fluorescence technique. Experimental results indicated that the lipase activity was inhibited by ILs and the degree of inhibition highly depended on the chemical structures of ILs. The inhibitory ability of the Cl- and Br--based ILs increased with increasing the alkyl chain length in the IL cation. Thermodynamic paremeters, enthalpy change (ON) and entropy change (AS) were obtained by analyzing the fluorescence behavior of lipase with the addition of ILs. Both Delta H and AS were positive suggesting hydrophobicity was the major driven force for the Cl-- and Br--based ILs. For the BF4-, CF3SO3-, ClO4- and N(CN)(2)(-)-based ILs, hydrogen bonding was the main driven force. For a more comprehensive understanding of the effects of ILs on lipase activity, the roles of hydrophobicity and hydrogen bonding must be considered simultaneously. A regression-based equation was developed to describe the relationship of the inhibitory ability of ILs and their hydrophobicity and hydrogen bonding ability. (C) 2016 Elsevier B.V. All rights reserved.