Sendai virus efficiently infects cells via the asialoglycoprotein receptor and requires the presence of cleaved F-0 precursor proteins for this alternative route of cell entry
Sendai virus efficiently infects cells via the asialoglycoprotein receptor and requires the presence of cleaved F-0 precursor proteins for this alternative route of cell entry
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DOI：
10.1128/jvi.71.7.5481-5486.1997
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发表时间：
1997-07-01
影响因子：
5.4
通讯作者：
Neubert, WJ
中科院分区：
文献类型：
--
作者：
Bitzer, M;Lauer, U;Neubert, WJ
生化证据表明，脱唾液酸糖蛋白受体（ASGP-R）可以作为一种替代受体的温度敏感型仙台病毒（SV）的突变体。我们现在已经研究了SV野生型（SV-wt）菌株伏见的这种可能的替代感染途径，通过使用一对细胞系，其仅在ASGP-R表达方面不同。酶促破坏常规含唾液酸SV受体（SA-R）后的感染研究显示，只有ASGP-R表达细胞可以被SV-wt感染。在感染前，通过将细胞与ASGP-R特异性抗体孵育，可以完全阻断这种替代的细胞进入途径。此外，SV-F前体蛋白切割成亚基F-1和F-2是建立通过ASGP-R的感染所必需的，这表明在SV-wt与宿主细胞上的ASGP-R结合后融合介导的细胞进入。有趣的是，发现通过ASGP-R的感染与通过常规的含唾液酸的SV受体的感染几乎一样有效。一个可能的生理作用的ASGP-R介导的SV感染的途径进行了讨论。
Biochemical evidence suggests that the asialoglycoprotein receptor (ASGP-R) can be used as an alternative receptor for a temperature-sensitive Sendai virus (SV) mutant. We now have investigated this possible alternative route of infection for SV wild-type (SV-wt) strain Fushimi by using a pair of cell lines which differ only with regard to ASGP-R expression. Infection studies after enzymatic destruction of conventional sialic acid-containing SV receptors (SA-R) revealed that only ASGP-R-expressing cells could be infected by SV-wt. This alternative route of cell entry could be completely blocked by incubation of cells with ASGP-R-specific antibodies prior to infection. Furthermore, cleavage of SV-F, precursor protein into the subunits F-1 and F-2 was necessary to establish infection via ASGP-R, suggesting a fusion-mediated cell entry after binding off SV-wt to the ASGP-R on host cells, Interestingly, infection via ASGP-R was found to be nearly as efficient as infection via conventional sialic acid-containing SV receptors. A possible physiological role of the ASGP-R-mediated route of SV infection is discussed.