Evaluation of collagen nerve guide in facial nerve regeneration
Evaluation of collagen nerve guide in facial nerve regeneration
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胶原神经导管在面神经再生中的评价
DOI：
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发表时间：
1998
影响因子：
1.3
通讯作者：
K. Endo
中科院分区：
文献类型：
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作者：
A. Kitahara;Yoshihisa Suzuki;Y. Nishimura;Kyoko Suzuki;T. Kiyotani;Y. Takimoto;Tatsuo Nakamura;Y. Shimizu;K. Endo
由于肿瘤切除或创伤导致的面神经麻痹是一种常见的并发症。因此，在本研究中，我们评估了胶原神经导管在跨 5 毫米神经间隙的面神经再生中的作用。该生物管由 3% 胶原蛋白制成，涂在仅用作模板的特氟隆管上，并在 105°C 下热脱水 24 小时。将胶原蛋白管植入 5 只成年猫的面神经背支，间隙为 5 毫米。对侧面神经保持完整，作为对照。术后3周开始进行电生理学研究，植入后8周进行组织学和辣根过氧化物酶标记检查。电生理学研究证实植入胶原的再生神经的电活动恢复。对植入胶原管的标本进行光学显微镜检查，发现血管化良好的再生神经，在电子显微镜下显示许多有髓鞘轴突被雪旺细胞和无髓鞘轴突包围。辣根过氧化物酶染色证明了脑干中的面部运动神经元和神经肌肉接头中的面部神经末梢的标记，也证实了整个面部神经束从重新神经支配的肌肉中恢复，通过再生部位到达脑干。胶原蛋白管作为 5 毫米面神经间隙的神经导管非常有效，并且作为神经导管显示出巨大的前景。
Facial nerve paralysis due to resection of tumors or as a consequence of trauma is a frequently observed complication. Thus, in the present study, we evaluated a collagen nerve guide in facial nerve regeneration across a 5-mm nerve gap. This biological tube was manufactured from 3% collagen, coated over a Teflon tube used only as a template and submitted to thermal dehydration at 105°C for 24h. The collagen tube was implanted at the dorsal ramous of the facial nerve of five adult cats over a gap of 5mm. The facial nerve of the contralateral side was kept intact and used as control. Electrophysiological study was performed from 3 weeks after surgery, and histological and horseradish peroxidase labeling examination was carried out 8 weeks after implantation. Electrophysiological study confirmed the recovery of electrical activity of the collagenimplanted regenerated nerve. Light-microscopic examination of collagen tube-implanted specimens revealed a well vascularized regenerated nerve, which under an electron microscope showed many myelinated axons surrounded by Schwann cells and unmyelinated axons. Horseradish peroxidase staining demonstrated labeling of facial motoneurons in the brainstem and facial nerve terminals in the neuromuscular junction, also confirming restoration of the whole facial nerve tract from the reinnervated muscles, passing through the regenerated site to the brainstem. The collagen tube was very efficient as a nerve guide over a 5mm facial nerve gap and shows great promise as a nerve conduit.