Cerebellar climbing fibers convey behavioral information of multiplex modalities and form functional modules
Cerebellar climbing fibers convey behavioral information of multiplex modalities and form functional modules
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小脑攀爬纤维传递多种模式的行为信息并形成功能模块
DOI：
10.1101/2022.08.24.505210
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kitamura Kazuo
中科院分区：
文献类型：
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作者：
Ikezoe Koji;Hidaka Naoki;Manita Satoshi;Murakami Masayoshi;Tsutsumi Shinichiro;Isomura Yoshikazu;Kano Masanobu;Kitamura Kazuo
小脑攀爬纤维（CFs）传递感觉运动信息及其错误，用于运动控制和学习。此外，它们还代表了与奖励相关的信息。尽管CF信号具有这样的功能多样性，但目前尚不清楚每个CF传递的是单一模式还是多种模式的信息，以及传递不同信息的CF如何分布在小脑皮层上。我们利用小脑浦肯野细胞（PCs）进行双光子钙成像，发现93%的PCs的CF反应可以通过多种行为变量的组合来解释，如杠杆运动、舔舐和奖励传递。相邻pc具有相似的CF响应特性，形成功能簇，响应噪声波动共享。综上所述，单个CFs在多重变量上传递行为信息，并在空间上组织成小脑皮层的功能模块。
Cerebellar climbing fibers (CFs) convey sensorimotor information and their errors, which are used for motor control and learning. Furthermore, they represent reward-related information. Despite such functional diversity of CF signals, it is still unclear whether each CF conveys the information of single or multiple modalities and how the CFs conveying different information are distributed over the cerebellar cortex. We performed two-photon calcium imaging from cerebellar Purkinje cells (PCs) in mice engaged in a voluntary forelimb lever-pull task and demonstrated that CF responses in 93% of PCs could be explained by the combination of multiple behavioral variables, such as lever movement, licking, and reward delivery. Neighboring PCs exhibited similar CF response properties, formed functional clusters, and shared noise fluctuations of responses. Taken together, individual CFs convey behavioral information on multiplex variables and are spatially organized into the functional modules of the cerebellar cortex.