Alcoholic hepatitis versus non-alcoholic steatohepatitis: Levels of expression of some proteins involved in tumorigenesis.
Alcoholic hepatitis versus non-alcoholic steatohepatitis: Levels of expression of some proteins involved in tumorigenesis.
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DOI：
10.1016/j.yexmp.2017.12.007
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发表时间：
2018-03
影响因子：
3.6
通讯作者：
French S
中科院分区：
文献类型：
--
作者：
Nguyen L;Masouminia M;Mendoza A;Samadzadeh S;Tillman B;Morgan T;French B;French S
非酒精性脂肪性肝炎（NASH）通常与肥胖、2型糖尿病和/或高脂血症相关，而酒精性脂肪性肝炎（ASH）与酒精滥用相关。如果不治疗，NASH和ASH患者都可能发展为肝硬化和肝细胞癌（HCC）。然而，NASH和ASH的肿瘤发生率似乎不同。患有NAS H的个体以每年0.5%的速率进展为HCC（Lindenmeyer等人，2017年），当患有ASH的个体以每年3-10%的速度进展为HCC时。因此，我们研究的目的是确定NASH与ASH在参与癌症发展的不同蛋白质表达水平上是否存在差异。使用的方法是使用荧光抗体的免疫组织化学染色测量NASH和ASH患者的肝活检切片中表达的蛋白质，然后用形态计量法定量荧光强度。测试的20种蛋白质是癌症分子机制的免疫经典途径的一部分，包括：RAP 2B，NAIP，FYN，PAK 6，SUV 39 H1，GNAI 1，BAX，E2 F3，CKDN 2B，BAK 1，BCL 2，DIABLO，RASGRF 2，GNA 15，PIK 3CB，BRCA 1，MAP 2K 1，BIRC 3，CDK 2和ATM。在ASH中，表现出表达水平上调的蛋白质是SUV 39 H1、E2 F3、BCL 2、BAK 1、BIRC 3和GNAI 1。在NASH中，表达水平上调的蛋白质是BAK 1和GNAI 1，表达水平下调的蛋白质是BCL 2。此外，与NASH相比，SUV 39 H1、E2 F3、BCL 2、BAK 1、BIRC 3和GNAI 1的表达水平在ASH中显著上调。这些结果显示，与正常肝脏相比，ASH存在显着差异，与NASH相比，ASH存在显着差异。因此，我们得出结论，与NASH相比，ASH中有更多的蛋白质参与肿瘤发生，与正常肝脏相比，ASH中有更多的蛋白质参与肿瘤发生，这与ASH和NASH中已知的肿瘤发展速率一致。
Non-alcoholic steatohepatitis (NASH) is commonly associated with obesity, type 2 diabetes, and/or hypertriglyceridemia, while alcoholic steatohepatitis (ASH) is associated with alcohol abuse. Both NASH and ASH patients can develop cirrhosis and hepatocellular carcinoma (HCC) if left untreated. However, the rate of tumorigenesis in NASH and ASH appears to be different. Individuals with NAS H progress to HCC at a rate of 0.5% annually (Lindenmeyer et al., 2017), when individuals with ASH progress to HCC at a rate of 3–10% annually. Thus, the objective of our study is to determine if there are differences in NASH versus ASH in the levels of different proteins expressed involved in cancer development. The method used was measuring the proteins expressed in liver biopsied sections from NASH and ASH patients using immunohistochemical staining with fluorescent ant ibodies and then quantitating the fluorescence intensity morphometrically. The 20 proteins tested are parts of the Ingenuity Canonical Pathway of Molecular Mechanisms of Cancer and include: RAP2B, NAIP, FYN, PAK6, SUV39H1, GNAI1, BAX, E2F3, CKDN2B, BAK1, BCL2, DIABLO, RASGRF2, GNA15, PIK3CB, BRCA1, MAP2K1, BIRC3, CDK2, and ATM. In ASH, the proteins that showed upregulated levels of expression were SUV39H1, E2F3, BCL2, BAK1, BIRC3, and GNAI1. In NASH, the proteins that showed upregulated levels of expression were BAK1 and GNAI1 and the protein that showed downregulated level of expression was BCL2. Additionally, levels of expression for SUV39H1, E2F3, BCL2, BAK1, BIRC3, and GNAI1 were significant upregulated in ASH compared to NASH. These results showed significant differences in ASH compared to normal liver, and significant differences in ASH compared to NASH. Thus, we conclude that there are more proteins involved in tumorigenesis in ASH compared to NASH and in ASH compared to normal liver, which is consistent with the known tumor development rate in ASH and NASH.
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影响因子：
64.5
作者：
Zender, Lars;Spector, Mona S.;Lowe, Scott W.
通讯作者：
Lowe, Scott W.
影响因子：
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作者：
Yao J;Liang LH;Zhang Y;Ding J;Tian Q;Li JJ;He XH
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作者：
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Leone, Gustavo
DOI：
10.1097/00022744-200209000-00004
发表时间：
2002-09-01
影响因子：
1.6
作者：
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通讯作者：
Cohen, C
影响因子：
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作者：
Lin, YW;Chen, CH;Sheu, JC
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