Tissue distribution of messenger ribonucleic acid encoding the rat glucagon-like peptide-1 receptor
Tissue distribution of messenger ribonucleic acid encoding the rat glucagon-like peptide-1 receptor
复制标题
DOI：
10.1210/en.137.7.2968
复制
发表时间：
1996-07-01
期刊：
影响因子：
4.8
通讯作者：
Habener, JF
中科院分区：
文献类型：
--
作者：
Bullock, BP;Heller, RS;Habener, JF
胰促胰岛素激素胰高血糖素样肽-1 （GLP-1）是餐后胰岛素分泌的重要调节因子。除了对胰岛β细胞的促胰岛素作用外，GLP-1还通过胰岛素不依赖的机制增强葡萄糖的处理，这表明GLP-1受体位于胰腺外组织。本研究采用RNAse保护、RT-PCR和原位杂交等方法检测大鼠GLP-1受体（GLP-1R）信使RNA （mRNA）在组织中的分布。通过RNAse保护实验，我们在肺、胰岛、胃和肾脏中发现了GLP-1R mRNA。RT-PCR分析还检测到了GLP-1R mRNA在下丘脑和心脏的表达。原位杂交实验在胃的胃窝、肺的大有核细胞、十二指肠隐窝和胰岛中发现了受体mRNA。在肾脏、骨骼肌、心脏、肝脏或脂肪细胞中未发现局部特异性颗粒。这些结果表明，与克隆大鼠胰岛GLP-1受体相对应的序列在胰岛、肺、下丘脑、胃、心脏和肾脏中表达，但在脂肪、肝脏和骨骼肌中不表达。此外，在肾脏和心脏中表达的GLP-1受体可能是已知受体的结构变体。因此，观察到的GLP-1的胰腺外作用可能并不局限于与定义的GLP-1受体的相互作用。
The incretin hormone glucagon-like peptide-1 (GLP-1) is an important regulator of postprandial insulin secretion. In addition to its insulinotropic actions on pancreatic beta-cells, GLP-1 enhances glucose disposal by insulin-independent mechanisms, suggesting that GLP-1 receptors are located on extrapancreatic tissues. In this study, we examined the tissue distribution of GLP-1 receptor (GLP-1R) messenger RNA (mRNA) in rat by RNAse protection, RT-PCR, and in situ hybridization. We identified GLP-1R mRNA in the lung, pancreatic islets, stomach, and kidney by the RNAse protection assay. RT-PCR analysis also detected GLP-1R mRNA in the hypothalamus and heart. In situ hybridization experiments identified receptor mRNA in the gastric pits of the stomach, large nucleated cells in the lung, crypts of the duodenum, and pancreatic islets. No localized specific grains were found in kidney, skeletal muscle, heart, liver, or adipocytes. These results indicate that sequences corresponding to the cloned rat islet GLP-1 receptor are expressed in the pancreatic islets, lung, hypothalamus, stomach, heart, and kidney but not in adipose, liver, and skeletal muscle. Further, the GLP-1 receptor expressed in the kidney and heart may be structural variants of the known receptor. Therefore, the observed extrapancreatic actions of GLP-1 may not be strictly confined to interactions with the defined GLP-1 receptor.