Photodynamic therapy induces microRNA-210 and-296 expression in HeLa cells
Photodynamic therapy induces microRNA-210 and-296 expression in HeLa cells
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DOI：
10.1002/jbio.200900082
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发表时间：
2010-06-01
影响因子：
2.8
通讯作者：
Kushibiki, Toshihiro
中科院分区：
文献类型：
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作者：
Kushibiki, Toshihiro
凋亡相关的microRNAs（miRNAs），其表达水平在光动力疗法（PDT）后观察到增加，被鉴定为PDT功效的标志物。miRNA形成一类小的内源性RNA，其通过靶向mRNA进行切割或翻译抑制而发挥重要的调节作用。miRNA参与多种生物学过程，包括发育、细胞生长、凋亡和造血，这表明在癌症发展中的作用。然而，没有以前的报告描述了PDT后细胞凋亡和miRNA表达之间的关系。在这项研究中，通过实时PCR在HeLa细胞中PDT后表征了凋亡相关的miRNA水平。PDT后1小时，miR-210和miR-296的表达水平增加，但其他与乳腺癌相关的miRNA（miR-7、miR-148 a、miR-204和miR-216）的表达水平没有变化。由于miR-210是在缺氧条件下持续刺激的最显著的miRNA，并且VEGF能够增加miR-296表达水平，因此这些数据表明PDT诱导的缺氧诱导miR-210表达，随后是VEGF表达和miR-296表达的增加。这些结果构成了使用他拉泊芬钠作为光敏剂的PDT后miRNA表达分析的第一份报告，证明miR-210和miR-296表达水平是HeLa细胞中PDT功效的标志物。
Apoptosis-related microRNAs (miRNAs), the expression levels of which were observed to increase after photodynamic therapy (PDT), were identified as markers of PDT efficacy. miRNAs form a class of small endogenous RNAs that play important regulatory roles by targeting mRNA for cleavage or translational repression. miRNAs contribute to diverse biological processes, including development, cell growth, apoptosis, and hematopoiesis, which suggests a role in cancer development. However, no previous reports have described a relationship between apoptosis and miRNA expression after PDT. In this study, apoptosis-related miRNA levels were characterized by real-time PCR after PDT in He La cells. miR-210 and miR-296 expression levels increased 1 hour after PDT, although the other apoptosis-related miRNA (miR-7, miR-148a, miR-204, and miR-216) expression levels were unchanged. Since miR-210 is the most prominent miRNA consistently stimulated under hypoxic conditions and VEGF is capable of increasing miR-296 expression levels, these data suggest that hypoxia induced by PDT induces miR-210 expression, followed by an increase of VEGF expression and miR-296 expression. These results constitute the first report of an analysis of miRNA expression after PDT using talaporfin sodium as a photosensitizer, demonstrating that miR-210 and miR-296 expression levels are markers of PDT efficacy in HeLa cells.