Odontoblast response to cavitypreparation with Er:YAG laser in ratmolars: an immunohistochemicalstudy
Odontoblast response to cavitypreparation with Er:YAG laser in ratmolars: an immunohistochemicalstudy
复制标题
成牙本质细胞对大鼠磨牙中 Er:YAG 激光制备腔体的反应：一项免疫组织化学研究
DOI：
10.1007/s10266-012-0078-x
复制
发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Okiji T
中科院分区：
文献类型：
--
作者：
Shigetani Y;Suzuki H;Ohshima H,Yoshiba K;Yoshiba N;Okiji T
本研究旨在研究用铒钇铝石榴石（Er:YAG）激光制备大鼠磨牙腔后成牙细胞系细胞的动态变化。采用Er:YAG激光在8周龄Wistar大鼠上颌左第一磨牙近中表面制备腔体。对侧第一磨牙作为未辐照对照。在激光照射后6和12小时，1、2、3、5和7天（各n= 5），立即收集标本，进行热休克蛋白(HSP)-25和巢蛋白的免疫组织化学处理，作为成牙干细胞系细胞的标记物。采用溴脱氧尿苷（BrdU）标记进行细胞增殖试验。未辐照的牙齿在成牙细胞中显示热休克蛋白-25和巢蛋白免疫反应性。照射后6 ~ 12 h，成牙细胞层组织紊乱，部分成牙细胞失去对热休克蛋白25和巢蛋白的免疫反应性。然而，在1-2天，成牙细胞层中热休克蛋白25和巢蛋白的免疫反应性显示出明显的恢复，导致成牙细胞样细胞重排，在3-7天对热休克蛋白25和巢蛋白产生强烈的免疫反应。brdu阳性细胞在第2天显著增加（与前一个时间点比较，P< 0.05；单向方差分析和scheff<s:1>事后检验），在第3天达到峰值，然后显著下降（P< 0.05）。由此可见，在本实验条件下，Er:YAG激光造腔对大鼠磨牙成牙细胞造成轻度、可逆的损伤。修复过程的特征是热休克蛋白-25和巢蛋白免疫反应性成牙细胞样细胞的重排，这发生在成牙细胞层破坏之后，这些免疫反应性部分丧失。
This study aimed to examine the dynamics of odontoblast-lineage cells following cavity preparation with erbium:yttrium–aluminum-garnet (Er:YAG) laser in rat molars. Cavity preparation was made with Er:YAG laser in the mesial surface of the maxillary left first molar of 8-week-old Wistar rats. Contralateral first molar served as unirradiated control. Immediately, 6 and 12 h and 1, 2, 3, 5 and 7 days after the lasing (n= 5, each), specimens were collected and processed for immunohistochemistry for heat-shock protein (HSP)-25 and nestin as markers for odontoblast-lineage cells. Cell proliferation assay using bromodeoxyuridine (BrdU) labeling was also performed. Unirradiated teeth showed HSP-25- and nestin-immunoreactivity in odontoblasts. At 6–12 h after irradiation, the odontoblastic layer was disorganized and some of odontoblasts lost the immunoreactivity to HSP-25 and nestin. At 1–2 days, however, HSP-25- and nestin-immunoreactivities in the odontoblast layer showed a noticeable recovery, resulting in the rearrangement of odontoblast-like cells intensely immunoreactive to HSP-25 and nestin at 3–7 days. BrdU-positive cells showed a significant increase at 2 days (P< 0.05 vs. immediate previous time point; one-way analysis of variance and Scheffé post hoc test), peaked at 3 days and then decreased significantly (P< 0.05). It was concluded that under the present experimental condition in rat molars, cavity preparation with Er:YAG laser induced mild and reversible damage to odontoblasts. The reparative process was characterized by the rearrangement of HSP-25- and nestin-immunoreactive odontoblast-like cells, which took place subsequent to the odontoblastic layer disorganization with partial loss of these immunoreactivities.