Phenotypes of dnaA mutants of Escherichia coli sensitive to detergents and organic solvents.
Phenotypes of dnaA mutants of Escherichia coli sensitive to detergents and organic solvents.
复制标题
对洗涤剂和有机溶剂敏感的大肠杆菌 dnaA 突变体的表型。
DOI：
10.1006/bbrc.1995.1939
复制
发表时间：
1995
影响因子：
3.1
通讯作者：
K. Sekimizu
中科院分区：
文献类型：
--
作者：
T. Shinpuku;T. Mizushima;L. Guo;T. Miki;K. Sekimizu
我们研究了大肠杆菌的dnaA突变体的洗涤剂（脱氧胆酸，胆酸）和有机溶剂（乙醇，甘油）的生长敏感性。dnaA 602，dnaA 604，dnaA 5，和dnaA 46突变体与突变的DnaA蛋白的ATP结合位点的洗涤剂比野生型菌株表现出更高的敏感性。然而，在DnaA蛋白的N-末端区域具有突变的dnaA 508和dnaA 167突变体对有机溶剂表现出更高的敏感性。在DnaA蛋白的C-末端区域具有突变的dnaA 204和dnaA 205突变体显示与野生型菌株对试剂相似的敏感性。用携带野生型dnaA基因的质粒进行的互补分析证实，dnaA突变是对试剂敏感的这些表型的原因。
We examined growth sensitivities of dnaA mutants of Escherichia coli to detergents (deoxycholic acid, cholic acid) and organic solvents (ethanol, glycerol). The dnaA602, dnaA604, dnaA5, and dnaA46 mutants with mutations in the putative ATP binding site of DnaA protein showed higher sensitivities to the detergents than did the wild-type strain. The dnaA508 and dnaA167 mutants with mutations in the N-terminal region of DnaA protein, however, showed higher sensitivities to organic solvents. The dnaA204 and dnaA205 mutants with a mutation in the C-terminal region of DnaA protein showed similar sensitivity as did the wild-type strain to reagents. Complementation analysis with a plasmid carrying the wild-type dnaA gene confirmed that dnaA mutations are responsible for these phenotypes that are sensitive to the reagents.