Development of a CMS-specific marker based on chloroplast-derived mitochondrial sequence in pepper
Development of a CMS-specific marker based on chloroplast-derived mitochondrial sequence in pepper
复制标题
DOI：
10.1007/s11816-009-0103-x
复制
发表时间：
2009-10-01
影响因子：
2.4
通讯作者：
Kang, Byoung-Cheorl
中科院分区：
文献类型：
--
作者：
Jo, Yeong Deuk;Jeong, Hee-Jin;Kang, Byoung-Cheorl
利用两个与细胞质雄性不育相关的基因orf 456和psiatp 6 -2的侧翼序列开发的分子标记，已被用于辣椒细胞质雄性不育的分子标记辅助选择。然而，在实践中，orf 456和psi atp 6 -2在亚化学计量水平的存在，甚至在保持系阻碍了含有CMS基因的植物的可靠选择。本研究开发了一种新的CMS特异性分子标记accD-U，用于辣椒CMS系的可靠鉴定，并利用该标记对辣椒育种系和种质的细胞质类型进行了鉴定。该标记是从CMS辣椒品系的线粒体基因组中叶绿体衍生序列的缺失中开发的。CMS辣椒品系可以通过accD-U标记带的存在或不存在来明确地确定。orf 456、psiatp 6 -2和accD-U在辣椒育种中的应用表明，accD-U是最可靠的细胞质雄性不育选择标记。orf 456在辣椒细胞质中的广泛分布表明，含有orf 456和psi atp 6 -2的辣椒细胞质已从仅含有orf 456的细胞质中被选作CMS细胞质。此外，orf 456以外的因子可能是调控辣椒雄性不育所必需的。
Molecular markers developed from the flanking sequences of two cytoplasmic male sterility (CMS)-associated genes, orf456 and psi atp6-2, have been used for marker-assisted selection of CMS in pepper. However, in practice, the presence of orf456 and psi atp6-2 at substoichiometric levels even in maintainer lines hampers reliable selection of plants containing the CMS gene. In this study, we developed a novel CMS-specific molecular marker, accD-U, for reliable determination of CMS lines in pepper, and used the newly and previously developed markers to determine the cytoplasm types of pepper breeding lines and germplasms. This marker was developed from a deletion in a chloroplast-derived sequence in the mitochondrial genome of a CMS pepper line. CMS pepper lines could be unambiguously determined by presence or absence of the accD-U marker band. Application of orf456, psi atp6-2 and accD-U to various pepper breeding lines and germplasms revealed that accD-U is the most reliable CMS selection marker. A wide distribution of orf456, but not psi atp6-2, in germplasms suggests that the pepper cytoplasm containing both orf456 and psi atp6-2 has been selected as CMS cytoplasm from cytoplasm containing only orf456. Furthermore, factors other than orf456 may be required for the regulation of male sterility in pepper.