Characterisation of follicular dendritic cells in labial salivary glands of patients with primary Sjogren syndrome: Comparison with tonsillar lymphoid follicles
Characterisation of follicular dendritic cells in labial salivary glands of patients with primary Sjogren syndrome: Comparison with tonsillar lymphoid follicles
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DOI：
10.1136/ard.56.2.140
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发表时间：
1997-02-01
影响因子：
27.4
通讯作者：
Wakefield, D
中科院分区：
文献类型：
--
作者：
Aziz, KE;McCluskey, PJ;Wakefield, D
目的-定位和表征原发性干燥综合征自身免疫病变中存在的滤泡树突状细胞 (FDC)。方法-通过间接免疫过氧化物酶技术和一组树突状细胞标记物的单克隆抗体对 15 名原发性干燥综合征患者的唇唾液腺低温切片进行检查。还检查了两个对照的扁桃体是否有相同的标记。结果-在唇唾液腺活检中，FDC 位于 75% 淋巴局灶结构的中心。原发性干燥综合征患者的唇唾液腺中的 FDC 表达 CD35、CD11c 和 CD106 (VCAM-1)，其模式与扁桃体中的 FDC 相似，但不表达 CD14 或 CD11b。这表明它们可能不是骨髓来源的，而扁桃体淋巴滤泡中的 FDC 强烈表达 CD14 和 CD11b。患者唇唾液腺中的 FDC 也缺乏扁桃体中 FDC 表达的 VLA-2 α 和 VLA-3 α。 结论：这些细胞的特征表型和起源可能对于干燥综合征的免疫反应和腺体中浸润淋巴细胞的保留具有重要意义。
Objective-To localise and characterise follicular dendritic cells (FDC) present in autoimmune lesions of primary Sjogren syndrome.Methods-Cryostat sections of labial salivary glands from 15 patients with primary Sjogren syndrome were examined by an indirect immunoperoxidase technique and monoclonal antibodies to a panel of dendritic cell markers. Tonsils from two controls were also examined for the same markers.Results-FDC were localised in the centre of 75% of lymphoid focal structures in labial salivary gland biopsies. FDC in labial salivary glands of patients with primary Sjogren syndrome expressed CD35, CD11c, and CD106 (VCAM-1) in a pattern similar to FDC in tonsils, but they did not express either CD14 or CD11b. This indicates that they may not be of myeloid origin, while FDC in tonsillar lymphoid follicles strongly expressed both CD14 and CD11b. FDC in labial salivary glands of patients also lacked VLA-2 alpha and VLA-3 alpha, which were expressed by FDC in tonsils.Conclusions-The characteristic phenotype and origin of these cells may be of importance in the immune responses involved in Sjogren syndrome and the retention of infiltrating lymphocytes in the glands.