The MR tracking of transplanted ATDC5 cells using fluorinated poly L-lysine-CF3
The MR tracking of transplanted ATDC5 cells using fluorinated poly L-lysine-CF3
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DOI：
10.1016/j.biomaterials.2006.08.050
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发表时间：
2007-01-01
期刊：
影响因子：
14
通讯作者：
Tooyama, Ikuo
中科院分区：
文献类型：
--
作者：
Maki, Junichi;Masuda, Chiaki;Tooyama, Ikuo
使用超顺磁性氧化铁(SPIO)的磁共振(MR)成像是监测活体动物移植细胞的强大工具。然而，由于SPIO是阴性造影剂，因此很难追踪最初显示低信号的骨和软骨中的移植细胞。在这项研究中，我们研究了异硫氰酸荧光素标记的聚L赖氨酸CF3(PLK-CF3)在小鼠ATDC5细胞(一种骨和软骨细胞系)上示踪的可行性。将FITC标记的PLK-CF3加入培养基易被ATDC5细胞内化。标记细胞植入小鼠颅骨至少7天，未见明显急性或长期毒性反应。FITC标记的PLK-CF3是一种有用的骨和软骨MR阳性对比剂。(C)2006爱思唯尔有限公司。保留所有权利。
Magnetic resonance (MR) imaging using super-paramagnetic iron oxides (SPIOs) is a powerful tool to monitor transplanted cells in living animals. However, since SPIOs are negative contrast agents it is difficult to track transplanted cells in bone and cartilage that originally display low signals. In this study, we examined the feasibility of tracking with fluorescein isothiocyanate (FITC)-labeled poly-L-lysine-CF3 (PLK-CF3) using mouse ATDC5 cells, a stein cell line of bone and cartilage cells. FITC-labeled PLK-CF3 was easily internalized by ATDC5 cells by adding it into culture medium. No acute or long-term toxicities were seen at less than 160 mu g/ml. Labeled cells transplanted into the cranial bone of mice were detected for at least 7 days by MR images. FITC-labeled PLK-CF3 is a useful positive contrast agent for MR tracking in bone and cartilage. (c) 2006 Elsevier Ltd. All rights reserved.