DISTINCTIVE PROPERTIES OF SIGNAL SEQUENCES FROM BACTERIAL LIPOPROTEINS
DISTINCTIVE PROPERTIES OF SIGNAL SEQUENCES FROM BACTERIAL LIPOPROTEINS
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DOI：
10.1093/protein/2.1.15
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发表时间：
1988-04-01
期刊：
影响因子：
--
通讯作者：
LAU, PCK
中科院分区：
文献类型：
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作者：
KLEIN, P;SOMORJAI, RL;LAU, PCK
我们已经比较了一些属性（疏水性，氨基酸大小，电荷和二级结构倾向）的信号序列的细菌脂蛋白与相同属性的信号肽从其他原核蛋白（非脂蛋白）。脂蛋白前导序列倾向于更短、更疏水和更庞大，并且它们具有更强的构象偏好，最显著的是预测的β-包含成熟蛋白的位置2或3的转角。另一个显著特征是位置-1和+2之间的局部能量分布中的最大值。除了一个例外（β-在脂蛋白（例如，内酰胺酶III）中，脂蛋白在其信号肽中不具有Pro，并且它们倾向于具有比非脂蛋白更少的Ser和Thr但更多的Gly。脂蛋白在成熟蛋白的N-末端区域也缺乏净负电荷。细菌素质粒编码的裂解蛋白的信号肽似乎是独特的，因为它们具有脂蛋白信号的所有归因特征;这些特征可用于指导信号肽诱变实验和构建新的分泌载体。
We have compared a number of attributes (hydrophobicity, amino acid size, charge and secondary structure propensities) of signal sequences of bacterial lipoproteins with the same attributes of signal peptides from other prokaryotic proteins (non-lipoproteins). Lipoprotein leader sequences tend to be shorter, more hydrophobic and bulky, and they have stronger conformational preferences, the most conspicuous being a predicted .beta.-turn comprising position 2 or 3 of the mature protein. Another distinctive feature is a maximum in the local energy profile between positions -1 and +2. With one exception (.beta.-lactamase III), the lipoproteins do not have Pro in their signal peptides, and they tend to have fewer Ser and Thr but more Gly than non-lipoproteins. Lipoproteins also lack a net negative charge in the N-terminal regions of the mature proteins. The signal peptides of the bacteriocin plasmid-coded lysis proteins appear to be unique in that they have all the ascribed features of lipoprotein signals; these characteristics can be used to guide signal peptide mutagenesis experiments and to construct new secretion vehicles.