The prebiotic, oligofructose-enriched inulin modulates the faecal metabolite profile: An in vitro analysis
The prebiotic, oligofructose-enriched inulin modulates the faecal metabolite profile: An in vitro analysis
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DOI：
10.1002/mnfr.201000136
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发表时间：
2010-12-01
影响因子：
5.2
通讯作者：
Verbeke, Kristin
中科院分区：
文献类型：
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作者：
De Preter, Vicky;Falony, Gwen;Verbeke, Kristin
适用范围：益生元给药的健康益处主要是从结肠中双歧杆菌数量的增加和短链脂肪酸产量的增加来判断的。只有少数研究集中在益生元的能力，以减少蛋白水解发酵，这可能有助于health.Methods和结果：益生元低聚果糖富集菊粉（OF-IN）的挥发性有机化合物的模式的影响，其特征在于使用体外粪便模型。将从健康受试者获得的粪便浆在37 ℃下与和不与不同剂量的OF-IN（2.5、5、10或20 mg）厌氧孵育，并评价代谢物模式和pH的变化。共鉴定了107种不同的挥发性有机化合物，并根据其化学类别进行了分类。随着OF-IN剂量的增加，酯和酸的浓度显著增加。对于一些醛，观察到类似的效果。相反，OF-IN剂量依赖性地抑制S-化合物的形成。此外，其他蛋白质发酵代谢产物，如酚类化合物的产生被抑制在OF-IN的存在下。结论：我们的研究结果证实了一个明确的剂量依赖性刺激糖解发酵。重要的是，有毒蛋白质发酵代谢物如硫化物的显著减少伴随着这些影响。
Scope: Health benefits of prebiotic administration have been judged mainly from the increased numbers of bifidobacteria and the enhanced production of short-chain fatty acids in the colon. Only a few studies have focused on the capacity of prebiotics to decrease the proteolytic fermentation, which might contribute to health as well.Methods and results: The influence of the prebiotic oligofructose-enriched inulin (OF-IN) on the pattern of volatile organic compounds was characterized using an in vitro faecal model. Faecal slurries, obtained from healthy subjects, were anaerobically incubated at 37 degrees C with and without different doses of OF-IN (2.5, 5, 10, or 20 mg) and changes in the metabolite pattern and pH were evaluated. A total of 107 different volatile organic compounds were identified and classified according to their chemical classes. The concentration of esters and acids significantly increased with increasing doses of OF-IN. Similar effects were observed for some aldehydes. To the contrary, OF-IN dose-dependently inhibited the formation of S-compounds. Also, the generation of other protein fermentation metabolites such as phenolic compounds was inhibited in the presence of OF-IN.Conclusion: Our results confirmed a clear dose-dependent stimulation of saccharolytic fermentation. Importantly, a significant decrease in toxic protein fermentation metabolites such as sulphides attended these effects.