CYTOPATHIC ASTROVIRUS ISOLATED FROM PORCINE ACUTE GASTROENTERITIS IN AN ESTABLISHED CELL-LINE DERIVED FROM PORCINE EMBRYONIC KIDNEY
CYTOPATHIC ASTROVIRUS ISOLATED FROM PORCINE ACUTE GASTROENTERITIS IN AN ESTABLISHED CELL-LINE DERIVED FROM PORCINE EMBRYONIC KIDNEY
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DOI：
10.1128/jcm.28.2.201-206.1990
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发表时间：
1990-02-01
影响因子：
9.4
通讯作者：
YAMANE, T
中科院分区：
文献类型：
--
作者：
SHIMIZU, M;SHIRAI, J;YAMANE, T
在一种已建立的细胞系中，从急性腹泻猪中分离到一种细胞性星形病毒，该细胞系是在胰酶的帮助下从猪胚胎肾脏中分离出来的。该病毒表现出明显的细胞病变效应，其特征是细胞增大，细胞质中出现细小颗粒。根据5-碘-2‘-脱氧尿嘧啶对其复制的影响，猪星状病毒有一个RNA基因组和5个多肽，分子量分别为13,000，30,000，31,000，36,000和39,000道尔顿，并且对脂类溶剂和50℃加热都是稳定的。C保存30分钟，但对酸(pH 3.0)有一定的不稳定性。该分离株在CSCL中的浮力密度为1.35g/ml，从疫区养殖场的腹泻猪中获得的成对血清标本对该病毒有明显的血清转化作用。对随机采集的8个猪群128头成年猪的血清标本进行中和试验，结果表明，50份血清标本中有50份猪星状病毒抗体阳性，但不同猪群之间的感染率存在较大差异，从0%到83%不等。子宫切除、缺乏初乳、4天龄的猪在口服暴露于细胞培养物中繁殖的猪星状病毒后出现轻度腹泻；从腹泻粪便标本中再次分离到该病毒。
A cytopathic astrovirus was isolated from pigs with acute diarrhea in an established cell line that was derived from porcine embryonic kidneys with the aid of trypsin. The virus showed a distinct cytopathic effect characterized by an enlargement of cells and the appearance of fine granules in the cytoplasm. Porcine astrovirus was shown to have an RNA genome, as determined by the effect of 5-iodo-2''-deoxyurine on its replication, and five polypeptides with molecular masses of 13,000, 30,000, 31,000, 36,000, and 39,000 daltons; and it was shown to be stable to lipid solvents and heating at 50.degree. C for 30 min but somewhat labile to acid (pH 3.0). The buoyant density of the isolate determined in CsCl was 1.35 g/ml. Seroconversion to the virus was evident in the paired serum specimens obtained from pigs with diarrhea that were housed at the farm where the disease occurred. The neutralization test on serum specimens collected randomly from 128 adult pigs of eight herds revealed that 50 of the serum specimens were positive for antibody to porcine astrovirus, although there was considerable variation in the prevalence among herds, ranging from 0 to 83%. Hysterectomy-produced, colostrum-deprived, 4-day-old pigs developed mild diarrhea after oral exposure to porcine astrovirus propagated in the cell culture; and the virus was isolated again from diarrheal stool specimens.