DEPENDENCY ON MEDIUM AND TEMPERATURE OF CELL SIZE AND CHEMICAL COMPOSITION DURING BALANCED GROWTH OF SALMONELLA-TYPHIMURIUM
DEPENDENCY ON MEDIUM AND TEMPERATURE OF CELL SIZE AND CHEMICAL COMPOSITION DURING BALANCED GROWTH OF SALMONELLA-TYPHIMURIUM
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DOI：
10.1099/00221287-19-3-592
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发表时间：
1958-01-01
期刊：
影响因子：
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通讯作者：
KJELDGAARD, NO
中科院分区：
文献类型：
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作者：
SCHAECHTER, M;MAALOE, O;KJELDGAARD, NO
研究了鼠伤寒沙门氏菌在不同培养基中平衡生长时的细胞质量、平均细胞核数、RNA和DNA含量。这些量可以被描述为在给定温度下由各种培养基提供的生长速率的指数函数。培养温度对培养基的大小和化学组成特性没有影响。因此，在平衡生长的条件下，这种生物体以大量可能的稳定生理状态之一存在。质量/细胞的变化是由于细胞核/细胞数量以及质量/细胞核数量的变化。核糖核蛋白颗粒数量的增加，在较高的增长率，它似乎，在很大程度上占质量/核的增加。计算表明，每单位RNA的蛋白质合成速率在所有生长速率下几乎相同。
Cell mass, the average number of nuclei/cell and the content of RNA and DNA were studied in Salmonella typhimurium during balanced (steady state) growth in different media. These quantities could be described as exponential functions of the growth rates afforded by the various media at a given temperature. The size and chemical composition characteristic of a given medium were not influenced by the temperature of cultivation. Thus, under conditions of balanced growth, this organism exists in one of a large number of possible stable physiological states. The variations in mass/cell are due to changes in the number of nuclei/ cell as well as in mass/nucleus. An increase in the number of ribonucleoprotein particles at higher growth rates could, it appears, largely account for the increase in mass/nucleus. Calculations indicate that the rate of protein synthesis per unit RNA is nearly the same at all growth rates.