Maternal obesity is associated with gut microbial metabolic potential in offspring during infancy
Maternal obesity is associated with gut microbial metabolic potential in offspring during infancy
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DOI：
10.1007/s13105-017-0577-x
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发表时间：
2018-02-01
影响因子：
3.4
通讯作者：
Campoy, Cristina
中科院分区：
文献类型：
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作者：
Cerdo, Tomas;Ruiz, Alicia;Campoy, Cristina
肥胖母亲所生的孩子患肥胖症的风险增加，但这种关联背后的机制尚未完全了解。我们的研究旨在调查18个月大的婴儿从早期婴儿喂养过渡到固体家庭食物时，微生物组编码的功能差异。为了研究母亲孕前体重指数对婴儿肠道微生物组的影响，通过16S rRNA基因测序和基于功能推断的微生物组分析来分析出生体重正常的婴儿（n = 21）和肥胖母亲（n = 18）的粪便样本。我们的研究结果表明，厚壁菌门显着丰富的婴儿出生体重正常的母亲，而拟杆菌显着丰富的婴儿出生肥胖的妇女。在这两种微生物组中，被分配功能的基因（> 50%）编码的蛋白质参与1级KEGG Orthology（KO）类别中的“代谢”。在较低KO功能类别下，体重正常母亲所生婴儿的微生物组的特征在于“戊糖磷酸途径”（p = 0.037）、“赖氨酸生物合成”（p = 0.043）、“甘油脂质代谢”（p = 0.042）和“C5分支二元酸代谢”（p = 0.045）的丰度显著富集。值得注意的是，肥胖母亲所生婴儿的微生物组在“链霉素生物合成”（p = 0.047）、“硫代谢”（p = 0.041）、“牛磺酸和亚牛磺酸代谢”（p = 0.036）和“脂多糖生物合成”（p = 0.043）方面显著富集。总之，我们的研究表明，母亲孕前肥胖可能会在婴儿后期留下具有不同功能表现的选择性肠道微生物组成。
Children born to obese mothers are at increased risk for obesity, but the mechanisms behind this association are not fully understood. Our study aimed to investigate differences in the functions encoded by the microbiome of infants at 18 months of age when the transition from early infant-feeding to solid family foods is established. To investigate the impact of maternal prepregnancy body mass index on infants' gut microbiome, faecal samples from infants born to normoweight (n = 21) and obese mothers (n = 18) were analysed by 16S rRNA gene sequencing and a functional-inference-based microbiome analysis. Our results indicated that Firmicutes was significantly enriched in infants born to normoweight mothers whereas Bacteroidetes was significantly enriched in infants born to obese women. In both microbiomes, the greatest number of genes (> 50%) that were assigned a function encoded for proteins involved in "metabolism" among tier 1 KEGG Orthology (KO) categories. At lower KO functional categories, the microbiome of infants born to normoweight mothers was characterized by a significant enrichment in the abundances of "pentose phosphate pathway" (p = 0.037), "lysine biosynthesis" (p = 0.043), "glycerolipid metabolism" (p = 0.042), and "C5-branched dibasic acid metabolism" (p = 0.045). Notably, the microbiome of infants born to obese mothers was significantly enriched in "streptomycin biosynthesis" (p = 0.047), "sulphur metabolism" (p = 0.041), "taurine and hypotaurine metabolism" (p = 0.036), and "lipopolysaccharide biosynthesis" (p = 0.043). In summary, our study showed that maternal prepregnancy obesity may imprint a selective gut microbial composition during late infancy with distinct functional performances.