A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling.
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发育程序截断长转录本以暂时调节细胞信号传导。
DOI：
10.1016/j.devcel.2018.11.019
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发表时间：
2018
影响因子：
11.8
通讯作者：
Stathopoulos,Angelike
中科院分区：
文献类型：
--
作者：
Sandler,JeremyE;Irizarry,Jihyun;Stepanik,Vincent;Dunipace,Leslie;Amrhein,Henry;Stathopoulos,Angelike
快速的有丝分裂和固定的转录速率限制了果蝇早期胚胎转录的最大长度。以前的研究表明，长基因的转录是启动的，但由于早期的核分裂有短的间期，所以被终止了。在这里，我们将在短核周期中表达的长基因识别为截短转录本。RNA结合蛋白Sex-Thal在物理上与这些基因的转录本相关，需要支持提前终止，以指定两性早期胚胎中较短的转录物异构体。此外，基因短缩-原肠融合基因的一个截短转录本编码胚胎中的一种产物，该产物在功能上与先前描述的显性负向形式有关，该形式维持转化生长因子-β信号处于关闭状态。综上所述，我们的结果揭示了一个短转录本的发育程序，它的功能是帮助暂时调节果蝇胚胎的发育，将早期阶段的细胞信号保持在最低限度，以支持其在细胞化时的适当启动。
Rapid mitotic divisions and a fixed transcription rate limit the maximal length of transcripts in earlyDrosophilaembryos. Previous studies suggested that transcription of long genes is initiated but aborted, as early nuclear divisions have short interphases. Here, we identify long genes that are expressed during short nuclear cycles as truncated transcripts. The RNA binding protein Sex-lethal physically associates with transcripts for these genes and is required to support early termination to specify shorter transcript isoforms in early embryos of both sexes. In addition, one truncated transcript for the geneshort-gastrulationencodes a product in embryos that functionally relates to a previously characterized dominant-negative form, which maintains TGF-β signaling in the off-state. In summary, our results reveal a developmental program of short transcripts functioning to help temporally regulateDrosophilaembryonic development, keeping cell signaling at early stages to a minimum in order to support its proper initiation at cellularization.