Isolation and characterization of an ammonium-oxidizing iron reducer: Acidimicrobiaceae sp. A6.

Isolation and characterization of an ammonium-oxidizing iron reducer: Acidimicrobiaceae sp. A6.
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DOI:
10.1371/journal.pone.0194007
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发表时间:
2018
期刊:
影响因子:
3.7
通讯作者:
Jaffé PR
Jaffé PR
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Huang S;Jaffé PR

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酸微菌科A6菌株(美国模式培养物保藏中心,专利保藏编号PTA - 122488),此前据报道在铁还原条件下对铵(NH₄⁺)的氧化起关键作用,现已从美国新泽西州的河岸湿地土壤中分离出来。在含有水铁矿作为三价铁[Fe(III)]源、二氧化碳作为碳源、室温以及pH值为4.5的培养基中对该菌株进行培养,在20天的培养期内,NH₄⁺的去除率达到52%,同时当NH₄⁺以Fe(III)作为电子受体被氧化为亚硝酸盐时,Fe(III)按预期的化学计量比被还原。这项研究表明,这种新分离的菌株能够在厌氧条件下氧化NH₄⁺同时还原铁。
Acidimicrobiaceae sp. A6 (ATCC, PTA-122488), a strain that has been previously reported to play a key role in the oxidation of ammonium (NH4+) under iron reducing conditions, has now been isolated from riparian wetland soils in New Jersey, USA. Incubations of this strain in a medium containing ferrihydrite as the ferric iron [Fe(III)] source, CO2 as the carbon source, under room temperature, and a pH of 4.5, resulted in 52% of NH4+ removal over a 20-day incubation period, while reducing Fe(III) in the expected stoichiometric ratio when NH4+ was oxidized to nitrite with Fe(III) as the electron acceptor. This study demonstrates that this new isolated strain is capable of oxidizing NH4+ while reducing iron under anaerobic conditions.
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