The Adrenal Lipid Droplet is a New Site for Steroid Hormone Metabolism
The Adrenal Lipid Droplet is a New Site for Steroid Hormone Metabolism
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肾上腺脂滴是类固醇激素代谢的新场所
DOI：
10.1002/pmic.201800136
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发表时间：
2018-12-01
期刊：
影响因子：
3.4
通讯作者：
Liu，Pingsheng
中科院分区：
文献类型：
--
作者：
Yu，Jinhai;Zhang，Linqiang;Liu，Pingsheng
类固醇激素对生物体起着至关重要的作用。内质网（ER）和线粒体是类固醇激素生物合成的重要场所，因为几种类固醇生成酶位于这些细胞器中，这一点已经被长期和公认。分析了人、猕猴和啮齿类动物肾上腺脂滴（LD）蛋白质组，揭示了类固醇生成酶也大量存在于LD上。发现的酶包括3β-羟基类固醇脱氢酶（HSD 3B）和雌二醇17β-脱氢酶11（HSD 17 B11）。Western blot和亚细胞定位分析一致表明，HSD 3B 2定位于LD。此外，体外实验证实，从稳定表达HSD 3B 2的HeLa细胞或从大鼠肾上腺分离的LD具有将双烯醇酮转化为孕酮的能力。总的来说，这些数据表明LD可能是类固醇激素代谢的重要位点。这些发现可能为类固醇激素的生物合成和代谢以及肾上腺疾病治疗的发展带来新的见解。
Steroid hormones play essential roles for living organisms. It has been long and well established that the endoplasmic reticulum (ER) and mitochondria are essential sites for steroid hormone biosynthesis because several steroidogenic enzymes are located in these organelles. The adrenal gland lipid droplet (LD) proteomes from human, macaque monkey, and rodent are analyzed, revealing that steroidogenic enzymes are also present in abundance on LDs. The enzymes found include 3β‐hydroxysteroid dehydrogenase (HSD3B) and estradiol 17β‐dehydrogenase 11 (HSD17B11). Analyses by Western blot and subcellular localization consistently demonstrate that HSD3B2 is localized on LDs. Furthermore, in vitro experiments confirm that the isolated LDs from HeLa cell stably expressing HSD3B2 or from rat adrenal glands have the capacity to convert pregnenolone to progesterone. Collectively, these data suggest that LDs may be important sites of steroid hormone metabolism. These findings may bring novel insights into the biosynthesis and metabolism of steroid hormones and the development of treatments for adrenal disorders.