Effects of Chymotrypsin on the Release Reaction and Aggregation of Blood Platelets
Effects of Chymotrypsin on the Release Reaction and Aggregation of Blood Platelets
复制标题
胰凝乳蛋白酶对血小板释放反应和聚集的影响
DOI：
10.3181/00379727-165-40943
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发表时间：
1980
影响因子：
--
通讯作者：
M. Zucker
中科院分区：
文献类型：
--
作者：
R. Grant;M. Zucker
用凝乳胰蛋白酶孵育和洗涤的血小板已知与纤维蛋白原聚集。据报道，凝乳胰蛋白酶可切割血小板膜糖蛋白，而不引起某些蛋白水解酶（如胰蛋白酶）所见的释放反应。两家制造商最近生产的几批凝乳胰蛋白酶导致血小板释放出其致密颗粒中的大部分内容物，主要是因为它们被胰蛋白酶污染了。然而，即使用N-α- toyl -l -赖氨酸氯甲基酮盐酸（TLCK）抑制胰蛋白酶后，凝乳胰蛋白酶在低浓度电离钙的情况下仍能诱导血小板释放一些钙离子。正如预期的那样，用胰蛋白酶污染或tlck处理的凝乳胰蛋白酶孵育的血小板在洗涤后与纤维蛋白原聚集。释放或添加ADP增强了纤维蛋白原的释放反应和聚集性的发展。因此，500 μg/ml胰凝乳蛋白酶对释放的ADP进行酶促破坏后，血小板中[14C]血清素的分泌和纤维蛋白原诱导的聚集减少，而在125 μg/ml胰凝乳蛋白酶对血小板进行酶促破坏前，添加25 μM ADP可使血清素分泌和纤维蛋白原诱导的聚集增加。
Abstract Platelets incubated with chymotrypsin and washed are known to aggregate with fibrinogen. Chymotrypsin is reported to cleave platelet membrane glycoproteins without causing the release reaction seen with some proteolytic enzymes such as trypsin. Recent batches of chymotrypsin from two manufacturers caused platelets to release much of the contents of their dense granules, largely because they are contaminated with trypsin. However, even after inhibition of trypsin with N-α-tosyl-L-lysine chloromethylketone hydrochloride (TLCK), chymotrypsin induced some release from platelets at low concentrations of ionized calcium. As expected, platelets incubated with either trypsin-contaminated or TLCK-treated chymotrypsin aggregated with fibrinogen after they were washed. Released or added ADP enhanced the release reaction and development of aggregability with fibrinogen. Thus, [14C]serotonin secretion and fibrinogen-induced aggregation were less when released ADP was enzymatically destroyed during incubation of platelets with 500 μg/ml of trypsin-contaminated chymotrypsin, and they were increased if 25 μM ADP was added before incubating the platelets with 125 μg/ml of this chymotrypsin.