First detection and characterisation of Eimeria zaria in European chickens
First detection and characterisation of Eimeria zaria in European chickens
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欧洲鸡中艾美耳球虫的首次检测和表征
DOI：
10.1016/j.vetpar.2023.110068
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发表时间：
2023
影响因子：
2.6
通讯作者：
Jaramillo-Ortiz J
中科院分区：
文献类型：
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作者：
Jaramillo-Ortiz J
近几十年来，全球家禽业经历了急剧的增长，增加了鸡病原体的重要性。艾美球虫属的原生动物寄生虫引起球虫病，损害动物健康和福利，并产生显著的年度成本。七种艾美球虫长期以来被认为会感染鸡，2007/8年首次从澳大利亚报道的三种新候选物种补充了这一点。这些新物种被命名为Eimeria lata、Eimeria nagambie和Eimeria扎里亚，在澳大利亚、撒哈拉以南非洲的几个国家、印度、委内瑞拉以及最近在美利坚合众国都有报告，但在欧洲没有发现。在法国、希腊、意大利、荷兰、爱尔兰共和国和英国，在生产的最后一周对一组56个未接种疫苗的肉鸡养殖场进行了采样，以使用特异性聚合酶链反应（PCR）评估所有十种艾美球虫的发生率。总的来说，56个农场中有39个（69.6%）发现至少有一个物种。tenella和E. maxima最常见，E. MitisandE.在法国的一个农场发现了腐败艾美耳球虫，而E.在欧洲首次发现了扎氏艾美耳球虫，出现在希腊和意大利（各出现一次）。设计了新的引物，以确认检测E。zaria，为与澳大利亚参考菌株进行系统发育比较提供了模板。检测E.鉴于目前的抗球虫疫苗缺乏保护作用，欧洲zariain强调了综合控制球虫病的重要性。
The global poultry industry has experienced dramatic growth in recent decades, increasing the significance of pathogens of chickens. Protozoan parasites of the genusEimeriacan cause the disease coccidiosis, compromising animal health and welfare, and incurring significant annual costs. SevenEimeriaspecies have long been recognised to infect chickens, supplemented by three new candidate species first reported from Australia in 2007/8. NamedEimeria lata,Eimeria nagambieandEimeria zaria, one or more of these new species have been reported in Australia, several countries in sub-Saharan Africa, India, Venezuela, and most recently the United States of America, but none have been detected in Europe. Here, a panel of 56 unvaccinated broiler chicken farms were sampled in the final week of production from France, Greece, Italy, the Netherlands, the Republic of Ireland, and the United Kingdom to assess the occurrence of all tenEimeriaspecies using specific polymerase chain reaction (PCR). Overall, 39 of 56 (69.6%) farms were found to host at least one species.Eimeria acervulina,E. tenella, andE. maximawere most common, withE. mitisandE. praecoxalso widespread.Eimeria necatrixwas detected on one farm in France, whileE. brunettiwas not detected.Eimeria zariawas detected for the first time in Europe, appearing in Greece and Italy (one occurrence each). New primers were designed to confirm detection ofE. zariaand provide template for phylogenetic comparison with the reference isolate from Australia. Detection ofE. zariain Europe reinforces the importance of integrated control for coccidiosis given the lack of protection induced by current anticoccidial vaccines.