HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY OF CARBOHYDRATES USING A NEW RESIN AND PULSED AMPEROMETRIC DETECTION
HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY OF CARBOHYDRATES USING A NEW RESIN AND PULSED AMPEROMETRIC DETECTION
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DOI：
10.1007/bf02688078
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发表时间：
1995-11-01
期刊：
影响因子：
1.7
通讯作者：
BONN, GK
中科院分区：
文献类型：
--
作者：
CORRADINI, C;CORRADINI, D;BONN, GK
本文报道了一种新的聚合物为基础的，强阴离子交换，固定相用于快速和选择性分离的碳水化合物和相关化合物的高pH值，阴离子交换色谱脉冲安培检测的研究结果。新的吸附剂已获得通过直接硝化的2.8 μ m，球形无孔高度交联，苯乙烯-二乙烯基苯共聚物珠，然后通过减少表面引入的硝基与新生氢和季铵化的所得氨基与碘甲烷。据报道，通过优化移动的相的离子强度，填充有新的阴离子交换剂的柱可以成功地用于分离，无论是在等度或梯度洗脱模式，寡糖，位置异构体的葡萄糖二糖，以及糖醛酸和糖单磷酸。
This paper reports the results of a study on the use of a new polymer-based, strong anion-exchange, stationary phase for rapid and selective separation of carbohydrates and related compounds by high-pH, anion-exchange chromatography with pulsed amperometric detection. The new adsorbent has been obtained by direct nitration of 2.8 mu m, spherical non-porous highly crosslinked, styrene-divinylbenzene copolymer beads, followed by reduction of superficially introduced nitro groups with nascent hydrogen and quaternization of the resultant amino groups with iodomethane. It is reported that by optimizing the ionic strength of the mobile phase, columns packed with the new anion-exchanger can be successfully employed to separate, either in isocratic or gradient elution mode, oligosaccharides, positional isomers of gluco-disaccharides, as well as uronic acids and sugar monophosphates.